Single-cell epigenetic landscape, microenvironment interactions, and gene regulatory modules of non-functioning pituitary adenomas (non-diseased controls, snMultiome, multiplexed)

The epigenetic landscape and tumor microenvironment (TME) interactions of non-functioning pituitary adenomas (NFPAs), benign tumors with high morbidity and recurrence rates, are not well characterized. We completed single-nucleus (sn) multiomics assays on four gonadotrope NFPAs. We identified genes involved in bidirectional tumor-macrophage crosstalk. We uncovered coordinated gene expression and chromatin accessibility programs that distinguished adenoma cells from gonadotropes. Integrated transcriptome-chromatin modeling revealed gene regulatory circuits that showed altered activity in adenoma cells. Our study provides insight into the altered epigenetic gene-control landscape and TME processes of the NFPA tumor phenotype.