Paired-end RNA Sequencing on primary human umbilical endothelial cells (HUVEC) exposed to uninfected or bacterially-infected PMA-differentiated macrophage-like U937 for 8 or 24 h
RNA-seq (gene expression) · human · 24 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Samples
- 24 samples: 24 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Listeria-infected macrophages promote biomechanical alterations in endothelial cell monolayers for transmigrationCell reports · 2026
Read the full summary ↓
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE296345_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1258956 and SRA study SRP583142. Searching any of these in the dataset finder brings you back here.
Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.
How we read this: Read from 24 sample titles: 24 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8968868 HUVEC exposed to Listeria monocytogenes-infected PMA-differentiated U937 for 8 h, A
- GSM8968869 HUVEC exposed to Listeria monocytogenes-infected PMA-differentiated U937 for 8 h, B
- GSM8968870 HUVEC exposed to Listeria monocytogenes-infected PMA-differentiated U937 for 8 h, C
- GSM8968871 HUVEC exposed to Listeria monocytogenes-infected PMA-differentiated U937 for 8 h, D
- GSM8968872 HUVEC exposed to Listeria monocytogenes-infected PMA-differentiated U937 for 24 h, A
- GSM8968873 HUVEC exposed to Listeria monocytogenes-infected PMA-differentiated U937 for 24 h, B
- GSM8968874 HUVEC exposed to Listeria monocytogenes-infected PMA-differentiated U937 for 24 h, C
- GSM8968875 HUVEC exposed to Listeria monocytogenes-infected PMA-differentiated U937 for 24 h, D
- GSM8968876 HUVEC exposed to no U937 for 8 h, A
- GSM8968877 HUVEC exposed to no U937 for 8 h, B
- GSM8968878 HUVEC exposed to no U937 for 8 h, C
- GSM8968879 HUVEC exposed to no U937 for 8 h, D
- GSM8968880 HUVEC exposed to no U937 for 24 h, A
- GSM8968881 HUVEC exposed to no U937 for 24 h, B
- GSM8968882 HUVEC exposed to no U937 for 24 h, C
- GSM8968883 HUVEC exposed to no U937 for 24 h, D
- GSM8968884 HUVEC exposed to PMA-differentiated U937 for 8 h,A
- GSM8968885 HUVEC exposed to PMA-differentiated U937 for 8 h, B
- GSM8968886 HUVEC exposed to PMA-differentiated U937 for 8 h, C
- GSM8968887 HUVEC exposed to PMA-differentiated U937 for 8 h, D
- GSM8968888 HUVEC exposed to PMA-differentiated U937 for 24 h, A
- GSM8968889 HUVEC exposed to PMA-differentiated U937 for 24 h, B
- GSM8968890 HUVEC exposed to PMA-differentiated U937 for 24 h, C
- GSM8968891 HUVEC exposed to PMA-differentiated U937 for 24 h, D
- GSE312719 Single-cell RNA sequencing analysis of HUVEC in monolayer alone or exposed to (bacterially-infected) macrophage-like U937 cells for 8 h. 9 samples shares huvec, u937, bacterially-infected, macrophage-like
- GSE275227 Transcriptomic changes in Human Umbilical Vein Endothelial Cells (HUVEC) after exposure to Tumor Necrosis Factor-Alpha(TNF-a), AdipoRon, and Sphingosine Kinase Inhibitor (SKI-I) 12 samples shares huvec, endothelial
- GSE241023 Investigation of differentially expressed genes in subpopulations of adherent human umbilical vein endothelial cells (HUVECS) isolated after fluid shear stress compared to the whole population of endothelial cells 12 samples shares huvec, endothelial
- GSE328193 Multiomic profiling of vascular endothelial cell differentiation from human embryonic stem cells [HUVEC RNA-Seq] 10 samples shares huvec, endothelial
- GSE293304 Regulation of endothelial cell chromatin availability and transcription factor activity in arterial-venous specification - HUVEC FUCCI RNAseq 6 samples shares huvec, endothelial
- GSE293694 Transcriptomic Profiling of TNFα-Stimulated Human Umbilical Vein Endothelial Cells (HUVECs) With and Without Isoginkgetin (ISOGK) Pretreatment. 6 samples shares huvec, endothelial
- GSE255600 RNA-binding protein RPS27 and gene expression regulation in human umbilical vein endothelial cells (HUVECs) 6 samples shares huvec, endothelial
- GSE52212 Sex-specific transcriptional differences between non-treated male and female human umbilical vein endothelial cells (HUVEC) 10 samples shares huvec, endothelial
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.