← BioTransfer GEO Dataset Finder
GEO series

Fatty Acid Esters of Hydroxy Fatty Acids (FAHFA) Derivatives as Dual Modulators of Lipid Metabolism and Gut Microbiome in Obesity

GSE296368 Mus musculus (mouse) RNA-seq (gene expression) 24 samples Submitted 2026/06/18 Platform GPL28330
At a glance

RNA-seq (gene expression) · mouse · 24 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
24 samples: 8 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
A gut-liver lipid flux checkpoint mediates FAHFA protection from MASLDPharmacological research · 2026
Summary (written by the submitters)
Fatty Acid Esters of Hydroxy Fatty Acids (FAHFAs), endogenous lipids with anti-inflammatory and metabolic benefits, face challenges in clinical translation due to instability and high synthesis costs. This study introduces a scalable synthetic platform to engineer novel FAHFA derivatives with enhanced stability through bioisosteric modifications (e.g., amide, triazole bonds).
Read the full summary ↓
Screening identified 12-TAASA and 12-HDTZSA as lead compounds, demonstrating potent inhibition of lipid uptake in hepatic cells without compromising glucose absorption. In a diet-induced obesity mouse model, oral administration of these derivatives reduced weight gain, improved glucose tolerance, and attenuated ectopic lipid deposition, rivaling the efficacy of semaglutide. Mechanistically, 12-TAASA and 12-HDTZSA remodeled the gut microbiome by enriching SCFA producing taxa. 12-TAASA also suppressed intestinal lipid absorption via transcriptomic regulation of key genes. These findings position synthetic FAHFAs as dual-action therapeutics, targeting both host lipid metabolism and gut microbiota dysbiosis, offering a promising strategy for obesity and metabolic disorders.
Published in
A gut-liver lipid flux checkpoint mediates FAHFA protection from MASLD
Xie H, Cheng HS, Ng JXJ et al. · Pharmacological research 2026 · PMID 41485679 · doi:10.1016/j.phrs.2025.108085
This dataset
Open GSE296368 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE296368_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1258999 and SRA study SRP583200. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41485679) ↗
Study design
8 conditions, mostly in triplicate
12-HDTZSA (100 mg/kg; daily via oral gavage),… ×3 12-SAASA (100 mg/kg; daily via oral gavage), … ×3 12-SAHSA (100 mg/kg; daily via oral gavage), … ×3 12-TAASA (100 mg/kg; daily via oral gavage), … ×3 12-TAHSA (100 mg/kg; daily via oral gavage), … ×3 Vehicle (corn oil; daily via oral gavage), co… ×3 Vehicle (corn oil; daily via oral gavage), LI… ×3 Semaglutide (100 μg/kg twice weekly via subcu… ×3

Supports a between-group comparison across 24 samples.

How we read this: 8 replicated groups read from 24 sample titles; they account for 24 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.