RNA-sequencing of Oral Squamous Cell carcinoma cells, PE/CA-PJ41, treated with human plasma or platelet-rich-plasma (PRP)

Platelets are involved in tumor progression by influencing cancer cell behavior through molecular and cellular interactions. In this study, we investigated the transcriptional response of PE/CA-PJ41 OSCC cells upon exposure to human plasma or PRP. Three experimental conditions were analysed: control, treated with 10% human plasma and 10% Platelet-Rich Plasma (PRP). After 48h, total RNA was extracted, and libraries were generated using polyA selection prior to paired-end sequencing on the Illumina NovaSeq X Plus platform. Twelve samples in total (four biological replicates per condition) were analyzed. The aim of this study is to compare the impact of plasma and PRP on gene expression in OSCC PJ41 cells, and to identify transcriptional changes specifically associated with platelets. This dataset is intended to serve as a ressource for understanding how platelets shape the tumor microenvironment and influence OSCC cell behavior at the molecular level.