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Investigations into the basal neural-like properties of dental pulp stem cells reveals they possess a functional type 2 muscarinic receptor which regulates quiescence

GSE297021 Homo sapiens (human) RNA-seq (gene expression) 16 samples Submitted 2025/09/24 Platform GPL24676
At a glance

RNA-seq (gene expression) · human · 16 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
16 samples: 4 × DPSCs, control, 4hrs, Biol vs 4 × DPSCs, control, 24hrs, Biol vs 4 × DPSCs, APE, 4hrs, Biol vs 4 × DPSCs, APE, 24hrs, BiolGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Investigations into the basal neural-like properties of dental pulp stem cells reveal they possess a functional type 2 muscarinic receptor which regulates quiescenceStem cell research & therapy · 2025
Summary (written by the submitters)
Dental pulp stem cells (DPSCs) are a population of mesenchyme-derived cells residing within the dental pulp known for their multipotent differentiation potential and neural-like properties. While a functional cholinergic system has been described in various mesenchymal stem cell (MSC) populations, its presence and role in DPSCs remain unexplored.
Read the full summary ↓
This study investigated the neurotransmitter-associated gene expression profile of human DPSCs using a targeted array panel and immunocytochemistry. The functionality of acetylcholine receptors (AChRs) was assessed through pharmacological activation and inhibition using receptor-specific agonists and antagonists. Cellular responses to cholinergic modulation, including effects on viability and proliferation, were evaluated via LDH release, CCK-8, and annexin V/PI staining assays. Among the 92 neurotransmitter-associated genes profiled, 43 were expressed, including multiple muscarinic receptors and nicotinic receptor subunits. Protein level expression of the m2, m3, and m5 muscarinic receptors was confirmed. Furthermore, activation of the m2AChR, using arecaidine propargyl ester hydrobromide (APE), led to a reversible reduction in DPSC proliferation without compromising cell viability. The findings demonstrate that DPSCs exhibit cholinoceptive properties and identify the m2AChR as a key modulator of cell cycle arrest. This receptor may serve as a therapeutic target for regulating DPSC behaviour and maintaining stem cell quiescence in regenerative applications.
Published in
Investigations into the basal neural-like properties of dental pulp stem cells reveal they possess a functional type 2 muscarinic receptor which regulates quiescence
Alqahtani S, Gibbs A, Verma M et al. · Stem cell research & therapy 2025 · PMID 41225501 · doi:10.1186/s13287-025-04730-7
This dataset
Open GSE297021 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE297021_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1262396 and SRA study SRP584891. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41225501) ↗
Study design
4 × DPSCs, control, 4hrs, Biol vs 4 × DPSCs, control, 24hrs, Biol vs 4 × DPSCs, APE, 4hrs, Biol vs 4 × DPSCs, APE, 24hrs, Biol

Supports a between-group comparison across 16 samples.

How we read this: 4 replicated groups read from 16 sample titles; they account for 16 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.