Targeting circulating mechanoresponsive monocytes and macrophages to reduce fibrosis
Single-cell RNA-seq · mouse · 12 samples
- What it is
- Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart. Read from the summary: GEO's own type field only says 'expression profiling by sequencing', so confirm it in the paper.
- Organism
- Mus musculus (mouse)
- Samples
- 12 samples: 12 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Targeting circulating mechanoresponsive monocytes and macrophages to reduce fibrosisNature biomedical engineering · 2026
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE297064_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.
Also filed as BioProject PRJNA1262462 and SRA study SRP585046. Searching any of these in the dataset finder brings you back here.
Supports a case/control comparison: 1 samples read as cases, 3 as controls.
How we read this: Read from 12 sample titles: 12 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8983033 Unwounded murine skin
- GSM8983034 Mechanically strained incision
- GSM8983035 Incision with no additional mechanical strain
- GSM8983036 Mechanically strained incision in wildtype mouse
- GSM8983037 Mechanically strained incision in Lyz2-Cre+Ptk2-flox knockout mouse
- GSM8983038 Incision with no additional mechanical strain from parabiotic mice GFP cells
- GSM8983039 Mechanically strained incision in wildtype mouse from parabiotic mice GFP cells
- GSM8983040 Mechanically strained incision in WT mouse from parabiotic mice with FAKI GFP cells
- GSM8983041 Co-culture of murine macrophages and fibroblasts in 3D collagen scaffold with mechanical strain
- GSM8983042 Same as above but also with FAKI treatment
- GSM8983043 Human skin processed with Visium spatial transcriptomics
- GSM8983044 Human scar processed with Visium spatial transcriptomics
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- GSE156337 Onco-fetal reprogramming of endothelial cells drives immunosuppressive macrophages in Hepatocellular Carcinoma 186 samples shares macrophages
- GSE314854 Targeting Modulated Vascular Smooth Muscle Cells in Atherosclerosis via FAP-Directed Immunotherapy 102 samples shares targeting
- GSE267740 Targeting T cell plasticity by pooled single cell CRISPR-screening in preclinical models of kidney and gut inflammation 56 samples shares targeting
- GSE333477 Dynamicity of Topoisomerase 1 binding at super-enhancers regulates TLR-responsive gene expression program in macrophages 106 samples shares macrophages
- GSE270148 Myeloid progenitor dysregulation fuels immunosuppressive macrophages in tumours 85 samples shares macrophages
- GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated 73 samples shares macrophages
- GSE255372 Targeting CDK7 function in the RNA Polymerase II transcription cycle in inflammatory arthritis [ChIP-seq] 69 samples shares targeting
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.