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Ephrin-B2 Deletion in GABAergic Neurons Induces Cognitive Deficits Associated with Single-Nucleus Transcriptomic Differences in the Prefrontal Cortex

GSE297542 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2025/05/31 Platform GPL24247
Summary
A total of 11,308 single nuclei were sequenced. After quality filtering (Methods, Supplementary Fig. 3A, 4A-4C), 11,090 high-quality nuclei were obtained, including 5,336 cells from EB2-flox mouse and 5,754 from EB2-vGATCre mouse. These nuclei were grouped into 17 cell clusters (Supplementary Fig. 4D-4H), which were further assigned to 9 major cell types based on cell-type-specific markers: excitatory neurons (Slc17a7+), inhibitory neurons (Gad2+), astrocytes (Gja1+), microglia (C1qa+), oligodendrocytes (Aspa+), oligodendrocyte precursors (Pdgfra+), smooth muscle cell (Uaca+), endothelial (Flt1+) and ependymal (Dynlrb2+) cells (Fig. 4A, 4C, supplementary Fig. 3B).
Published in
Ephrin-B2 deletion in GABAergic neurons induces cognitive deficits associated with single-nucleus transcriptomic differences in the prefrontal cortex
Ju P, Fang Y, Xiang S et al. · BMC biology 2025 · PMID 40764556 · doi:10.1186/s12915-025-02333-5
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Also filed as BioProject PRJNA1265165 and SRA study SRP586327. Searching any of these in the dataset finder brings you back here.

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