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Fluvastatin protects Leydig cells in orchitis through direct activation of Peroxiredoxin 1

GSE298181 Mus musculus (mouse) RNA-seq (gene expression) 18 samples Submitted 2025/12/03 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 18 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
18 samples: 6 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
Orchitis caused by infection is one of the most important causes of male infertility, which can cause an imbalance of the oxidation/antioxidation system. Appropriate antioxidant therapy may be effective in alleviating testicular damage. Previous studies found that peroxiredoxin 1 (PRDX1) is involved in the regulation of oxidative stress and inflammation, and is highly expressed in testis.
Read the full summary ↓
Therefore, selecting an effective PRDX1 agonist might become an attractive strategy in the treatment of orchitis related to oxidative stress. In the current study, we identified the effective agonist of PRDX1. Leydig cells were used to find the antioxidant role and mechanisms of the agonist in vitro. Models of orchitis in wild-type and Prdx1-/- mice were employed to confirm the anti-orchitis effect of the agonist. We found that fluvastatin (FV), a classical cholesterol-lowering drug, could directly bind PRDX1 to activate its enzyme activity. FV played a protective and antioxidant role on H2O2-treated Leydig cells by improving cell viability and testosterone level, inhibiting apoptosis, and reducing reactive oxygen species level and inflammatory factors in a PRDX1-dependent manner. FV could also decrease oxidative stress and inflammation levels of testes in mice models, thereby reducing the damage on testicular steroidogenesis and spermatogenesis caused by orchitis. FV was an effective agonist of PRDX1, which showed antioxidant and anti-inflammatory impact in orchitis. Our results may shed light on the antioxidant treatment of orchitis and male infertility.
This dataset
Open GSE298181 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE298181_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1268478 and SRA study SRP588083. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗
Study design
6 conditions, mostly in triplicate
Mus musculus, Leydig cells, siNC, biol ×3 Mus musculus, Leydig cells, siNC+H2O2, biol ×3 Mus musculus, Leydig cells, siNC+H2O2+FV, biol ×3 Mus musculus, Leydig cells, siprdx1, biol ×3 Mus musculus, Leydig cells, siprdx1+H2O2, biol ×3 Mus musculus, Leydig cells, siprdx1+H2O2+FV, … ×3

Supports a between-group comparison across 18 samples.

How we read this: 6 replicated groups read from 18 sample titles; they account for 18 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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