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Single Cell Glycome and Transcriptome Profiling Enabled by a Library of Anti-glycan Antibodies

GSE298512 Homo sapiens Expression profiling by high throughput sequencing; Other 8 samples Submitted 2026/05/20 Platform GPL24676Platform GPL18573
Summary
Despite their importance in cellular processes and as clinical targets, glycans remain difficult to study with existing technologies. A major barrier to developing tools to better study glycans is the dearth of high-quality, well-characterized anti-glycan antibodies. To create a resource for optimally selecting specific anti-glycan antibodies and understanding their general properties, we generated a publicly accessible database of 650 anti-glycan antibody sequences, recombinantly expressed a library of 154 of these antibodies, and characterized their binding properties using a glycan microarray. We DNA-barcoded a subset of these antibodies to create a platform enabling simultaneous high-throughput glycome and transcriptome profiling at the single-cell level. Using this platform, we profiled the two isogenic colorectal cancer cell lines, SW480 and SW620. The results reveal a variety of glycans associated with cancer stem cells and metastasis and demonstrate the power of integrating the glycome into multi-omic efforts to discover biomarkers and therapeutic targets.
Published in
Single-cell glycome and transcriptome profiling enabled by a library of anti-glycan antibodies
Marglous S, Bhakta AZ, Gillmann KM et al. · Cell chemical biology 2025 · PMID 41380686 · doi:10.1016/j.chembiol.2025.11.005
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Also filed as BioProject PRJNA1269585 and SRA study SRP588696. Searching any of these in the dataset finder brings you back here.

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