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Decoding glioblastoma invasion: single-cell profiling of environmental and cell cycle effects in tumour assembloids

GSE298718 Homo sapiens Expression profiling by high throughput sequencing 7 samples Submitted 2026/02/05 Platform GPL24676Platform GPL34284
Summary
Akhunbay-Fudge et al. develop two single-cell profiling techniques to dissect glioblastoma invasion phenotypes, focusing on the effects of lineage commitment (neural versus endodermal) and cell cycle progression within tumour assembloids. The study employs single-cell mRNA sequencing to compare tumour invasion into neural versus endodermal lineage assembloids and establishes the ‘DyPheT’ tracking algorithm to correlate cell cycle phases with malignant cell migration in cerebral organoids in real time. These complementary methods reveal glioblastoma-induced changes in environmental cells, identify cell-autonomous gene expression profiles of assembloid invasion in tumour cells, and emphasize a "go-and-grow" invasion paradigm, identifying highly migratory cells active during the S/G2 phase.
Published in
Glioblastoma invasion into different organoid hosts reveals cell-intrinsic and proliferative migratory programs
Akhunbay-Fudge CY, Irving BK, Ismail A et al. · iScience 2026 · PMID 41959666 · doi:10.1016/j.isci.2026.115361
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Also filed as BioProject PRJNA1271001 and SRA study SRP589283. Searching any of these in the dataset finder brings you back here.

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