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single cell chromatin accessiblity profiling in formlin fixed paraffin embedded samples

GSE299388 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 8 samples Submitted 2025/10/14 Platform GPL34328
Summary
We evaluated the performance of ATAC-seq (Assay for Transposase-Accessible Chromatin using sequencing) on formalin-fixed paraffin-embedded (FFPE) spleen samples from C57BL/6J mice. Nuclei were isolated from FFPE mouse spleen tissue, and ATAC-seq was performed at both the bulk and single-cell levels, with and without reverse crosslinking. The resulting datasets were compared to ATAC-seq and scATAC-seq profiles obtained from fresh spleen samples. Our analysis revealed a markedly reduced library complexity in FFPE-derived samples compared to fresh tissue, across both bulk and single-cell experiments. These findings highlight the technical limitations of applying standard ATAC-seq protocols to FFPE samples. Based on our results, we conclude that conventional ATAC-seq approaches are not well-suited for chromatin accessibility profiling in FFPE-preserved tissues.
Published in
scFFPE-ATAC enables high-throughput single cell chromatin accessibility profiling in formalin-fixed paraffin-embedded samples
Yadav RP, Xing P, Zhao M et al. · Nature communications 2025 · PMID 41238550 · doi:10.1038/s41467-025-66170-4
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Also filed as BioProject PRJNA1274238 and SRA study SRP591019. Searching any of these in the dataset finder brings you back here.

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