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scRNA-Seq of CD45+ cells in B16F10 tumor after adoptive cytotoxic T lymphocyte immunotherapy

GSE299448 Mus musculus Expression profiling by high throughput sequencing 3 samples Submitted 2026/04/26 Platform GPL28457
Summary
Although adoptive cell transfer (ACT) with tumor-specific CD8⁺ T cells induces initial tumor regression, tumors often relapse due to incomplete eradication and immune evasion. To investigate the mechanisms of tumor regrowth after ACT, we performed single-cell RNA sequencing (scRNA-seq) of tumor-infiltrating immune cells in a murine melanoma model. B16F10 melanoma-bearing C57BL/6 mice were treated with gp100-specific Pmel-1 cytotoxic T lymphocytes (CTLs). Tumors were harvested on days 3, 7, and 14 post-transfer, and CD45⁺ immune cells were isolated for 10x Genomics Chromium-based scRNA-seq. This dataset supports the analysis of immune cell states during tumor progression after ACT.
Published in
Mathematical modeling of immune counter-regulation predicts efficacy of fractionated CD8(+) T cell dosing strategies
Kasai H, Nagaoka K, Lysenko A et al. · Scientific reports 2026 · PMID 42062426 · doi:10.1038/s41598-026-50922-3
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Also filed as BioProject PRJNA1274460 and SRA study SRP591074. Searching any of these in the dataset finder brings you back here.

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