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Increased LEF1 protein levels and isoform switching drive cell proliferation in chronic lymphocytic leukemia [RNA-Seq]

GSE299964 Homo sapiens (human) RNA-seq (gene expression) 50 samples Submitted 2025/10/08 Platform GPL30173
At a glance

RNA-seq (gene expression) · human · 50 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
50 samples: 16 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Increased LEF1 protein levels and isoform switching drive cell proliferation in chronic lymphocytic leukemiaBlood · 2025
Summary (written by the submitters)
The main goal of this study was to assess the role of LEF1 in patient-derived CLL cells.
Published in
Increased LEF1 protein levels and isoform switching drive cell proliferation in chronic lymphocytic leukemia
Mateos-Jaimez J, Vidal-Crespo A, Charalampopoulou S et al. · Blood 2025 · PMID 40983031 · doi:10.1182/blood.2025030129
This dataset
Open GSE299964 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE299964_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1277728 and SRA study SRP592513. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40983031) ↗
Study design
16 conditions, mostly in duplicate
M-CLL3_Day ×2 M-CLL5_Day ×2 M-CLL9_Day ×2 M-CLL4_Day ×2 U-CLL11_Day ×2 U-CLL12_Day ×2 U-CLL2_Day ×2 U-CLL1_Day ×2 +26 more

Supports a between-group comparison across 32 samples.

How we read this: 16 replicated groups read from 50 sample titles; they account for 32 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 10 more — browse all 50 samples with per-sample file links →

Datasets on a similar topic

Search all human RNA-seq datasets in GEO →

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.