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Effect of PRLX-93936 treatment on gene expression of PANC-1 cells

GSE300405 Homo sapiens (human) RNA-seq (gene expression) 18 samples Submitted 2025/08/13 Platform GPL34281
At a glance

RNA-seq (gene expression) · human · 18 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
18 samples: 6 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Defining the Antitumor Mechanism of Action of a Clinical-stage Compound as a Selective Degrader of the Nuclear Pore ComplexCancer discovery · 2025
Summary (written by the submitters)
PRLX-93936 is an optimized derivative of erastin. To determine the downstream effects of PRLX-93936 on gene expression, we performed mRNAseq of PANC-1 cells treated with the drug or vehicle control.
Published in
Defining the Antitumor Mechanism of Action of a Clinical-stage Compound as a Selective Degrader of the Nuclear Pore Complex
Yuan L, Ji W, Dwyer BG et al. · Cancer discovery 2025 · PMID 40891634 · doi:10.1158/2159-8290.CD-25-0271
This dataset
Open GSE300405 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE300405_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1280899 and SRA study SRP593842. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40891634) ↗
Study design
6 conditions, mostly in triplicate
DMSO, 2hr, ×3 DMSO, 6hr, ×3 PRLX-93936 0.5uM, 2hr, ×3 PRLX-93936 0.5uM, 6hr, ×3 PRLX-93936 5uM, 2hr, ×3 PRLX-93936 5uM, 6hr, ×3

Supports a between-group comparison across 18 samples.

How we read this: 6 replicated groups read from 18 sample titles; they account for 18 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.