GEO series

Parental preconceptual α-cypermethrin exposure alters embryonic brain transcriptomics in mice: implications for autism spectrum disorder and stress vulnerability

GSE300410 Mus musculus (mouse) RNA-seq (gene expression) 76 samples Submitted 2025/08/22 Platform GPL24247

At a glance

RNA-seq (gene expression) · mouse · 76 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 76 samples
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.

Summary (written by the submitters)

Pyrethroid insecticides are widely used in agriculture and households, and their exposure can affect neurodevelopment. Few studies have evaluated how preconception parental exposure could also affect this process. To address knowledge gap, adult C57Bl6/J mice were gavaged daily with -cypermethrin at a human relevant low (0.3 mg/kg) or high (10 mg/kg) dose in corn oil for four weeks prior to conception.

Read the full summary ↓

Offspring embryonic day 16 dorsal forebrain was extracted for transcriptomic analysis. In offspring forebrains of exposed compared to unexposed parents, there was increasing number of differentially expressed genes (DEGs) from paternal (least) to maternal to both parent exposure (most). A dose dependent effect was observed in offspring forebrain for paternal and maternal preconceptual exposures. Maternal and both parent exposures led to upregulated genes in offspring brain for biological processes involved in translation with predicted activation of EIF4E, a gene associated with autism. In contrast, paternal exposure upregulated cell cycle related DNA damage signaling processes. After any parent exposure, there was upregulation of biological processes involved in mitochondria function and oxidative stress and a downregulation of neuronal and synaptic processes with predicted inhibition of BDNF signaling. Weighted gene correlation network analysis identified modules associated with different parent treatment that were over-represented with DEGs with similar functional signatures as found in DEG-related pathways. Importantly, DEGs in offspring forebrain after any parent exposure were over-represented with genes related to autism spectrum disorder (ASD) and stress vulnerability. The study highlights the potential contribution of preconception parental pyrethroid exposure to aberrant brain functioning.

This dataset

Open GSE300410 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE300410_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1280917 and SRA study SRP593846. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Samples in this study

The sample list for this study is not cached yet. Press Load samples and it will be fetched from NCBI.

+ 76 more — browse all 76 samples with per-sample file links →

Datasets on a similar topic

GSE218362 Paternal exposure alone induces alcohol-related birth defects in a mouse model: Implications of paternal drinking in FAS birth defects. 16 samples shares implications, exposure
GSE275434 Behavioral and Neurochemical Changes in Mice Induced by Low-level Lead Exposure: Implications for ADHD and Conduct Disorders 9 samples shares implications, exposure
GSE241089 First cleavage is a manifestation of the geometry of the unfertilized oocyte: implications for monozygotic twinning in mice. 219 samples shares implications
GSE115706 RNAseq of motor neurons and neurons with differential vulnerability isolated from a mouse model of spinal muscular atrophy using laser capture microdissection 168 samples shares vulnerability
GSE223323 Enterocyte like differentiation defines metabolic gene signatures of CMS3 colorectal cancers and provides therapeutic vulnerability 148 samples shares vulnerability
GSE330865 Multiorgan transcriptomics and circulating extracellular vesicle profiling reveal age-dependent systemic vulnerability to isoflurane anesthesia and surgery 120 samples shares vulnerability
GSE310453 Functional divergence of Capicua isoforms explains differential tissue vulnerability in neurological disease 70 samples shares vulnerability
GSE244538 RNAseq of motor neurons with differential vulnerability to ALS isolated from the SOD1G93A mouse model of ALS 54 samples shares vulnerability

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.