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Liver Single-Cell Atlas of MASH and Fibrosis Models in Mice

GSE300744 Mus musculus Expression profiling by high throughput sequencing 3 samples Submitted 2026/06/18 Platform GPL34328
Summary
Metabolic dysfunction-associated steatohepatitis (MASH) and liver fibrosis represent distinct yet interconnected pathological processes underlying chronic liver disease progression. While high-fat diet (HFD)-induced MASH models recapitulate metabolic inflammation, carbon tetrachloride (CCl₄)-induced models capture toxicant-driven fibrogenesis. Here, we performed single-cell RNA sequencing (scRNA-seq) on liver tissues from HFD-induced MASH and CCl₄-induced fibrosis mouse models to construct a high-resolution atlas of liver cellular heterogeneity. This work establishes a valuable resource for dissecting liver disease mechanisms, enabling targeted therapeutic discovery and offering a framework for integrating diverse preclinical models of chronic liver injury.
Published in
Hepatocyte BDNF Acts as a Novel Immune Checkpoint to Restrain TLR4-Mediated Acute Hepatitis
Zhu W, Cui Y, Zhou Y et al. · Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 · PMID 41881032 · doi:10.1002/advs.202521164
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Direct links to NCBI, no account and no request form: the whole study as GSE300744_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 3 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1282010 and SRA study SRP594519. Searching any of these in the dataset finder brings you back here.

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