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Different COVID-19 vaccines induce distinct reprogramming of stromal functions in draining lymph nodes

GSE300959 Mus musculus Expression profiling by high throughput sequencing 7 samples Submitted 2025/08/15 Platform GPL24247
Summary
The non-leukocytic stromal cells of lymph nodes critically regulate immune responsiveness. However, the effects of different SARS-CoV-2 vaccines on stromal cell biology are unknown. We used single-cell transcriptomics to study early responses of stromal cells in draining lymph nodes after immunizing mice with clinically used COVID-19 vaccines, namely Spikevax®, Comirnaty®, Vaxzevria® and Nuvaxovid™. We found that vaccinations lead to robust transcriptomic changes, including vaccine-selective ones, in the different lymph node stromal cell populations priming the lymph node for the upcoming adaptive immune response.
Published in
COVID-19 vaccine type controls stromal reprogramming in draining lymph nodes
Fair-Mäkelä R, Thorén P, Näsiaho J et al. · Science immunology 2025 · PMID 40815672 · doi:10.1126/sciimmunol.adr6787
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Also filed as BioProject PRJNA1282737 and SRA study SRP594947. Searching any of these in the dataset finder brings you back here.

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