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Single-cell RNA-seq of neutrophils from germ-free and specific-pathogen-free mice reveals gut-microbiota-driven heterogeneity and NETosis in acute pancreatitis

GSE301305 Mus musculus Expression profiling by high throughput sequencing 5 samples Submitted 2025/08/20 Platform GPL24247
Summary
We performed single-cell RNA sequencing (scRNA-seq) using the 10x Genomics platform to characterize pancreatic immune cell populations in germ-free (GF) and specific-pathogen-free (SPF) mice with cerulein-induced acute pancreatitis (AP). scRNA-seq analysis identified distinct neutrophil subpopulations influenced by gut microbiota presence. SPF mice showed enrichment of pro-inflammatory neutrophil clusters associated with increased neutrophil extracellular trap (NET) formation, whereas GF mice exhibited neutrophil subsets expressing reparative and protective signatures. Our data highlight the role of gut microbiota in shaping immune responses during AP.
Published in
Gut microbiota dysbiosis exacerbates acute pancreatitis via Escherichia coli-driven neutrophil heterogeneity and NETosis
Zou Y, Li N, Li X et al. · Gut microbes 2026 · PMID 41439481 · doi:10.1080/19490976.2025.2606480
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Also filed as BioProject PRJNA1284780 and SRA study SRP596589. Searching any of these in the dataset finder brings you back here.

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