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RiboTag and bulk RNA sequencing using AAV-shGpr151 and AAV-shGpr151 with AAV-e5'UTR infected sensory neurons.

GSE301325 Mus musculus (mouse) RNA-seq (gene expression) 12 samples Submitted 2025/09/01 Platform GPL24973
At a glance

RNA-seq (gene expression) · mouse · 12 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
12 samples: 3 × RiboTag, adult DRG neuron, shGpr151 (Input) vs 3 × RiboTag, adult DRG neuron, shGpr151 with e5'UTR (Input) vs 3 × RiboTag, adult DRG neuron, shGpr151 (IP) vs 3 × RiboTag, adult DRG neuron, shGpr151 with e5'UTR (IP)Groups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
After discovering that Gpr151 mRNA regulates the CSDE1-mediated rp-mRNA operon, we designed an engineered version of the 5'UTR of Gpr151 mRNA(e5'UTR), which has an enhanced interaction with CSDE1. To determine if this e5'UTR RNA can restore the down-regulated rp-mRNA operon formation in Gpr151 knockdowned neurons, we performed total RNA and RiboTag sequencing analysis using AAV-shGpr151 or AAV-shGpr151 with AAV-e5'UTR infected sensory neurons using RiboTag mice, RPL22HA+/+;Advillin-Cre+.
This dataset
Open GSE301325 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE301325_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1284803 and SRA study SRP596435. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗
Study design
3 × RiboTag, adult DRG neuron, shGpr151 (Input) vs 3 × RiboTag, adult DRG neuron, shGpr151 with e5'UTR (Input) vs 3 × RiboTag, adult DRG neuron, shGpr151 (IP) vs 3 × RiboTag, adult DRG neuron, shGpr151 with e5'UTR (IP)

Supports a between-group comparison across 12 samples.

How we read this: 4 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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