GEO series
Cell-intrinsic effect of IL-7R signaling of monocytes within C57BL/6J mouse ears infected with Leishmania major [bulk RNA-seq]
GSE228456
Mus musculus (mouse)
Single-cell RNA-seq
87 samples
Submitted 2026/03/20
Platform GPL17021
At a glance
Single-cell RNA-seq · mouse · 87 samples
- What it is
- Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart. Read from the summary: GEO's own type field only says 'expression profiling by sequencing', so confirm it in the paper.
- Organism
- Mus musculus (mouse)
- Samples
- 87 samples: 87 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
To determine the cell-intrinsic effect of IL-7R signaling, we sorted il7r-/- (CD45.2+) and WT (CD45.1+) monocytes as internal controls from the same Leishmania major (Lm) infected ears of 50:50 WT:il7r-/ -bone marrow chimera and performed population RNAseq of pools of 50-100 cells per population and infection site using the SMARTseq2 protocol.
This dataset
Download · which file do I need?
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
1 · Just have a look
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
2 · Re-analyse it
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE228456_RAW.tar (it can run to tens of gigabytes).
3 · Start from the raw reads
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.
Also filed as BioProject PRJNA949955 and SRA study SRP429940. Searching any of these in the dataset finder brings you back here.
Study design
87 samples, each with a distinct title — no replicated groups
Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.
How we read this: Read from 87 sample titles: 87 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
Samples in this study
- GSM7121046 High-proliferating L. major infected, IL7R-/- compartment of BMC, cell-pool#1 , Mouse#2 , Right ear [L2002739_H1KO2R]
- GSM7121047 High-proliferating L. major infected, IL7R-/- compartment of BMC, cell-pool#2 , Mouse#5 , Left ear [L2002747_H1KO5L]
- GSM7121048 High-proliferating L. major infected, IL7R-/- compartment of BMC, cell-pool#3 , Mouse#5 , Left ear [L2002815_H1KO5L]
- GSM7121049 High-proliferating L. major infected, IL7R-/- compartment of BMC, cell-pool#4 , Mouse#5 , Right ear [L2002754_H1KO5R]
- GSM7121050 High-proliferating L. major infected, IL7R-/- compartment of BMC, cell-pool#5 , Mouse#6 , Left ear [L2002762_H1KO6L]
- GSM7121051 High-proliferating L. major infected, IL7R-/- compartment of BMC, cell-pool#6 , Mouse#6 , Right ear [L2002770_H1KO6R]
- GSM7121052 High-proliferating L. major infected, IL7R-/- compartment of BMC, cell-pool#7 , Mouse#7 , Left ear [L2002778_H1KO7L]
- GSM7121053 High-proliferating L. major infected, IL7R-/- compartment of BMC, cell-pool#8 , Mouse#7 , Right ear [L2002786_H1KO7R]
- GSM7121054 High-proliferating L. major infected, IL7R-/- compartment of BMC, cell-pool#9 , Mouse#8 , Left ear [L2002794_H1KO8L]
- GSM7121055 High-proliferating L. major infected, IL7R-/- compartment of BMC, cell-pool#10 , Mouse#8 , Right ear [L2002802_H1KO8R]
- GSM7121056 High-proliferating L. major infected, wt compartment of BMC, cell-pool#1 , Mouse#2 , Right ear [L2002740_H1WT2R]
- GSM7121057 High-proliferating L. major infected, wt compartment of BMC, cell-pool#2 , Mouse#5 , Right ear [L2002755_H1WT5R]
- GSM7121058 High-proliferating L. major infected, wt compartment of BMC, cell-pool#3 , Mouse#6 , Left ear [L2002763_H1WT6L]
- GSM7121059 High-proliferating L. major infected, wt compartment of BMC, cell-pool#4 , Mouse#6 , Right ear [L2002771_H1WT6R]
- GSM7121060 High-proliferating L. major infected, wt compartment of BMC, cell-pool#5 , Mouse#7 , Left ear [L2002779_H1WT7L]
- GSM7121061 High-proliferating L. major infected, wt compartment of BMC, cell-pool#6 , Mouse#7 , Right ear [L2002787_H1WT7R]
- GSM7121062 High-proliferating L. major infected, wt compartment of BMC, cell-pool#7 , Mouse#8 , Left ear [L2002795_H1WT8L]
- GSM7121063 High-proliferating L. major infected, wt compartment of BMC, cell-pool#8 , Mouse#8 , Right ear [L2002803_H1WT8R]
- GSM7121064 High-proliferating L. major infected, wt compartment of BMC, cell-pool#9 , Mouse#5 , Left ear [L2002748_H1WT5L]
- GSM7121065 Low-proliferating L. major infected, IL7R-/- compartment of BMC, cell-pool#1 , Mouse#1 , Right ear [L2002729_LoKO1R]
- GSM7121066 Low-proliferating L. major infected, IL7R-/- compartment of BMC, cell-pool#2 , Mouse#2 , Left ear [L2002733_LoKO2L]
- GSM7121067 Low-proliferating L. major infected, IL7R-/- compartment of BMC, cell-pool#3 , Mouse#2 , Right ear [L2002737_LoKO2R]
- GSM7121068 Low-proliferating L. major infected, IL7R-/- compartment of BMC, cell-pool#4 , Mouse#2 , Right ear [L2002808_LoKO2R]
- GSM7121069 Low-proliferating L. major infected, IL7R-/- compartment of BMC, cell-pool#5 , Mouse#3 , Left ear [L2002741_LoKO3L]
- GSM7121070 Low-proliferating L. major infected, IL7R-/- compartment of BMC, cell-pool#6 , Mouse#5 , Left ear [L2002745_LoKO5L]
- GSM7121071 Low-proliferating L. major infected, IL7R-/- compartment of BMC, cell-pool#7 , Mouse#6 , Left ear [L2002760_LoKO6L]
- GSM7121072 Low-proliferating L. major infected, IL7R-/- compartment of BMC, cell-pool#8 , Mouse#6 , Right ear [L2002768_LoKO6R]
- GSM7121073 Low-proliferating L. major infected, IL7R-/- compartment of BMC, cell-pool#9 , Mouse#6 , Right ear [L2002809_LoKO6R]
- GSM7121074 Low-proliferating L. major infected, IL7R-/- compartment of BMC, cell-pool#10 , Mouse#7 , Left ear [L2002776_LoKO7L]
- GSM7121075 Low-proliferating L. major infected, IL7R-/- compartment of BMC, cell-pool#11 , Mouse#7 , Right ear [L2002784_LoKO7R]
- GSM7121076 Low-proliferating L. major infected, IL7R-/- compartment of BMC, cell-pool#12 , Mouse#7 , Right ear [L2002810_LoKO7R]
- GSM7121077 Low-proliferating L. major infected, IL7R-/- compartment of BMC, cell-pool#13 , Mouse#8 , Left ear [L2002792_LoKO8L]
- GSM7121078 Low-proliferating L. major infected, IL7R-/- compartment of BMC, cell-pool#14 , Mouse#8 , Right ear [L2002800_LoKO8R]
- GSM7121079 Low-proliferating L. major infected, IL7R-/- compartment of BMC, cell-pool#15 , Mouse#8 , Right ear [L2002811_LoKO8R]
- GSM7121080 Low-proliferating L. major infected, wt compartment of BMC, cell-pool#1 , Mouse#1 , Right ear [L2002730_LoWT1R]
- GSM7121081 Low-proliferating L. major infected, wt compartment of BMC, cell-pool#2 , Mouse#2 , Left ear [L2002734_LoWT2L]
- GSM7121082 Low-proliferating L. major infected, wt compartment of BMC, cell-pool#3 , Mouse#2 , Right ear [L2002738_LoWT2R]
- GSM7121083 Low-proliferating L. major infected, wt compartment of BMC, cell-pool#4 , Mouse#2 , Right ear [L2002812_LoWT2R]
- GSM7121084 Low-proliferating L. major infected, wt compartment of BMC, cell-pool#5 , Mouse#3 , Left ear [L2002742_LoWT3L]
- GSM7121085 Low-proliferating L. major infected, wt compartment of BMC, cell-pool#6 , Mouse#5 , Left ear [L2002746_LoWT5L]
+ 47 more — browse all 87 samples with per-sample file links →
Datasets on a similar topic
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- GSE226609 Mouse lung tissue RNA-Seq from C57Bl6 wild type and ALOX15 KO mice with and without house dust mite (HDM) exposure. 20 samples shares c57bl, rna-seq
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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.