GEO series

Effect of Meflin overexpression on the fibroblast transcriptome in a three-dimensional culture using cell accumulation technique

GSE301570 Homo sapiens Expression profiling by high throughput sequencing 8 samples Submitted 2026/01/11 Platform GPL34284

Summary

We previously reported that Meflin, encoded by the immunoglobulin superfamily containing leucine-rich repeat (ISLR) gene, is a marker for tumor-restraining cancer-associated fibroblasts. However, the functional roles of Meflin in fibroblasts remain unclear. To investigate the functional impact of Meflin expression in fibroblasts, RNA sequencing was performed on Meflin-overexpressing NHDF-Ad cells cultured under conditions that better mimic the in vivo tumor microenvironment, using a cell accumulation technique that accurately recapitulates the fibrotic stroma observed in aggressive human cancers. Meflin overexpression resulted in the downregulation of PDPN, a well-established marker of tumor-promoting CAFs, and upregulation of DPT and COL15A1, which are markers of universal fibroblasts that resemble normal fibroblasts and are considered a potential source of diverse fibroblast subtypes.These findings suggest that high-level expression of Meflin is crucial for skewing CAFs toward a more universal fibroblast–like state.

Published in

Increased Meflin Expression in Cancer-Associated Fibroblasts Restrains Tumor Cell Proliferation and Shapes Vessel-Rich Stroma in Triple-Negative Breast Cancer

Sakai A, Miyai Y, Shiraki Y et al. · The American journal of pathology 2026 · PMID 41638315 · doi:10.1016/j.ajpath.2026.01.006

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Also filed as BioProject PRJNA1285463 and SRA study SRP597837. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41638315) ↗

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