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CITEseq of hepatic CD4+ T cells from healthy mice and mice with steatohepatitis

GSE301733 Mus musculus Expression profiling by high throughput sequencing; Other 4 samples Submitted 2026/05/18 Platform GPL30172
Summary
Unresolved inflammation and fibrosis are the two key features of metabolic dysfunction-associated steatohepatitis (MASH), a progressive form of steatotic liver disease that can evolve into cirrhosis and liver cancer. To better understand the role of CD4⁺ T cells in the pathogenesis of MASH, we comprehensively characterized hepatic CD4⁺ T cells in murine MASH at a single-cell protein and transcriptional level. CITE-sequencing revealed a marked shift in intrahepatic CD4⁺ T-cell composition in MASH, with enrichment of Th1, regulatory, and cytotoxic CD4⁺ T cells. Transcriptomic profiling also identified Tnfrsf4 (OX40) upregulation in hepatic CD4⁺ T cells during MASH. Together, these studies provide a proteogenomic single-cell atlas for hepatic CD4⁺ T cells and uncover a CD4⁺ T cell-dependent immunopathogenic circuit as a promising immunotherapeutic target to alleviate MASH and liver fibrosis.
Published in
CD4+ T cells promote fibrosis during metabolic dysfunction-associated steatohepatitis
Valenzuela-Pérez L, Kim Lee HS, Bayer RL et al. · Hepatology (Baltimore, Md.) 2026 · PMID 42141897 · doi:10.1097/HEP.0000000000001772
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Direct links to NCBI, no account and no request form: the whole study as GSE301733_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1287495 and SRA study SRP599301. Searching any of these in the dataset finder brings you back here.

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