Single-cell profiling of human liver and peripheral-blood γδ T cells according to HCMV serostatus

This study generated single-cell transcriptomic and surface-protein datasets to examine the heterogeneity of human liver sinusoidal γδ T cells and their association with latent human cytomegalovirus infection. γδ T cells isolated from liver perfusates of healthy living donors were profiled using single-cell RNA sequencing with antibody-derived tags. The dataset includes Vγ9Vδ2 and non-Vγ9Vδ2 γδ T-cell populations from HCMV-seropositive and HCMV-seronegative donors and enables comparison of their transcriptional states, surface phenotypes, differentiation patterns, and TCR repertoires. Paired peripheral-blood γδ T-cell samples were additionally profiled for comparison with liver-derived populations.eir diverse functions.