Promoter mutagenesis and a massively parallel reporter screen of the MAPT locus identifies cis-regulatory elements and genetic variation effects
RNA-seq (gene expression) · human · 140 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Samples
- 140 samples: 29 conditions, mostly with about 5 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Promoter mutagenesis and a massively parallel reporter screen of the MAPT locus identifies cis-regulatory elements and genetic variation effectsbioRxiv : the preprint server for biology · 2026
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE325670_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1441453. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 134 samples.
How we read this: 29 replicated groups read from 140 sample titles; they account for 134 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM9575993 Mixed Neuron Culture, CRISPRi AAV Safe Harbor, replicate 1 Mixed Neuron Culture, C…
- GSM9575994 Mixed Neuron Culture, CRISPRi AAV Safe Harbor, replicate 2 Mixed Neuron Culture, C…
- GSM9575995 Mixed Neuron Culture, CRISPRi AAV Safe Harbor, replicate 3 Mixed Neuron Culture, C…
- GSM9575996 Mixed Neuron Culture, CRISPRi AAV Safe Harbor, replicate 4 Mixed Neuron Culture, C…
- GSM9575997 Mixed Neuron Culture, CRISPRi AAV Safe Harbor, replicate 5 Mixed Neuron Culture, C…
- GSM9575998 Mixed Neuron Culture, CRISPRi AAV Safe Harbor, replicate 6 Mixed Neuron Culture, C…
- GSM9575999 Mixed Neuron Culture, CRISPRi AAV Safe Harbor, replicate 7 Mixed Neuron Culture, C…
- GSM9576000 Mixed Neuron Culture, CRISPRi AAV Safe Harbor, replicate 8 Mixed Neuron Culture, C…
- GSM9576001 Mixed Neuron Culture, CRISPRi AAV Safe Harbor, replicate 9 Mixed Neuron Culture, C…
- GSM9576002 Mixed Neuron Culture, CRISPRi MAPT promoter, replicate 1 Mixed Neuron Culture, C…
- GSM9576003 Mixed Neuron Culture, CRISPRi MAPT promoter, replicate 2 Mixed Neuron Culture, C…
- GSM9576004 Mixed Neuron Culture, CRISPRi MAPT promoter, replicate 3 Mixed Neuron Culture, C…
- GSM9576005 Mixed Neuron Culture, CRISPRi MAPT promoter, replicate 4 Mixed Neuron Culture, C…
- GSM9576006 Mixed Neuron Culture, CRISPRi MAPT promoter, replicate 5 Mixed Neuron Culture, C…
- GSM9576007 Mixed Neuron Culture, CRISPRi MAPT promoter, replicate 6 Mixed Neuron Culture, C…
- GSM9576008 Mixed Neuron Culture, CRISPRi MAPT promoter, replicate 7 Mixed Neuron Culture, C…
- GSM9576009 Mixed Neuron Culture, CRISPRi MAPT promoter, replicate 8 Mixed Neuron Culture, C…
- GSM9576010 Mixed Neuron Culture, CRISPRi MAPT promoter, replicate 9 Mixed Neuron Culture, C…
- GSM9576011 Mixed Neuron Culture, CRISPRi region r1, replicate 1 Mixed Neuron Culture, C…
- GSM9576012 Mixed Neuron Culture, CRISPRi region r1, replicate 2 Mixed Neuron Culture, C…
- GSM9576013 Mixed Neuron Culture, CRISPRi region r1, replicate 3 Mixed Neuron Culture, C…
- GSM9576014 Mixed Neuron Culture, CRISPRi region r1, replicate 4 Mixed Neuron Culture, C…
- GSM9576015 Mixed Neuron Culture, CRISPRi region r1, replicate 5 Mixed Neuron Culture, C…
- GSM9576016 Mixed Neuron Culture, CRISPRi region r2, replicate 1 Mixed Neuron Culture, C…
- GSM9576017 Mixed Neuron Culture, CRISPRi region r2, replicate 2 Mixed Neuron Culture, C…
- GSM9576018 Mixed Neuron Culture, CRISPRi region r2, replicate 3 Mixed Neuron Culture, C…
- GSM9576019 Mixed Neuron Culture, CRISPRi region r2, replicate 4 Mixed Neuron Culture, C…
- GSM9576020 Mixed Neuron Culture, CRISPRi region r2, replicate 5 Mixed Neuron Culture, C…
- GSM9576021 Mixed Neuron Culture, CRISPRi region r3, replicate 1 Mixed Neuron Culture, C…
- GSM9576022 Mixed Neuron Culture, CRISPRi region r3, replicate 2 Mixed Neuron Culture, C…
- GSM9576023 Mixed Neuron Culture, CRISPRi region r3, replicate 3 Mixed Neuron Culture, C…
- GSM9576024 Mixed Neuron Culture, CRISPRi region r3, replicate 4 Mixed Neuron Culture, C…
- GSM9576025 Mixed Neuron Culture, CRISPRi region r3, replicate 5 Mixed Neuron Culture, C…
- GSM9576026 Mixed Neuron Culture, CRISPRi region r4, replicate 1 Mixed Neuron Culture, C…
- GSM9576027 Mixed Neuron Culture, CRISPRi region r4, replicate 2 Mixed Neuron Culture, C…
- GSM9576028 Mixed Neuron Culture, CRISPRi region r4, replicate 3 Mixed Neuron Culture, C…
- GSM9576029 Mixed Neuron Culture, CRISPRi region r4, replicate 4 Mixed Neuron Culture, C…
- GSM9576030 Mixed Neuron Culture, CRISPRi region r4, replicate 5 Mixed Neuron Culture, C…
- GSM9576031 Mixed Neuron Culture, CRISPRi region r5, replicate 1 Mixed Neuron Culture, C…
- GSM9576032 Mixed Neuron Culture, CRISPRi region r5, replicate 2 Mixed Neuron Culture, C…
+ 100 more — browse all 140 samples with per-sample file links →
- GSE289173 Single-cell eQTL analysis identifies genetic variation underlying metabolic dysfunction-associated steatohepatitis 48 samples shares identifies, variation
- GSE230520 Osteopontin drives neuroinflammation and cell loss in MAPT-N279K frontotemporal dementia patient neurons 61 samples shares mapt
- GSE326573 T-cell Multiomic Analysis Identifies Subsets and Mechanisms of Interaction with Epithelial Cells in Idiopathic Pulmonary Fibrosis 59 samples shares identifies
- GSE286518 Integrative CSF profiling identifies disease-specific immune responses in leptomeningeal disease 42 samples shares identifies
- GSE285246 Spatially resolved atlas of IPF fibroblastic foci identifies that PAK2 kinase activation is involved in fibrogenesis 12 samples shares identifies
- GSE254707 Single-cell transcriptomic analysis identifies infiltrating plasmacytoid dendritic cells in psoriasis epidermis 4988 samples shares identifies
- GSE307247 Deciphering the cis-regulatory code of alternative splicing in human cell lines 11 samples shares cis-regulatory
- GSE325907 Placental gene co-expression networks and mediation analysis identifies pathways and genes associated with birthweight 319 samples shares identifies
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.