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CRISPR-mediated deletion of Prdm12 alters H3K9me3 landscape in CD8+ T cells: a CUT&Tag study

GSE301909 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 5 samples Submitted 2025/08/20 Platform GPL24247
Summary
In this study, we used magnetic bead-based separation to isolate CD8+ T cells from OTI:Cas9 transgenic mice. These cells were then transfected with Cas9 ribonucleoprotein (RNP) targeting the Prdm12 gene or non-targeting control RNP via electroporation. After 72 hours, both edited (Prdm12 knockout) and control cells were collected for chromatin profiling of histone modification H3K9me3 using CUT&Tag sequencing. The aim of this study is to investigate how Prdm12 regulates the epigenetic landscape, particularly H3K9me3 distribution, in CD8+ T cells.
Published in
Prdm12 governs an epigenetic checkpoint linking neuroimmune cross-talk to CD8(+) T cell exhaustion-suppressed antitumor immunity
Liu G, Tian X, Wang Q et al. · Science advances 2025 · PMID 40815657 · doi:10.1126/sciadv.adx9221
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Also filed as BioProject PRJNA1287732 and SRA study SRP598953. Searching any of these in the dataset finder brings you back here.

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