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scRNA-Seq of Retinal microglia from 6 months old Balb/c mice infected with MCMV at birth

GSE302157 Mus musculus Expression profiling by high throughput sequencing 3 samples Submitted 2026/01/29 Platform GPL24247
Summary
We used a single cell sequencing approach using 10x Genomics scRNAseq to understand the transcriptional differences between microglia from MCMV infected animals vs controls. MCMV infection at birth resulted in increased retinal degeneration with age. Microglia from mice infected with MCMV appeared to have an alternative activation state compared to age matched uninfected controls. We utilized a depletion and repopulation strategy to reset the population of microlgia using PLX5622, a CSFR1 inhibitor. This study allowed analysis of the transcriptional changes caused by MCMV infection early in life potentially contributing to neurodegeneration.
Published in
Persistent microglial activation following neonatal CMV infection mediates neurodegeneration
McCord JL, Chatterjee D, Han JYS et al. · Science advances 2026 · PMID 41811959 · doi:10.1126/sciadv.adz1686
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Also filed as BioProject PRJNA1289094 and SRA study SRP599614. Searching any of these in the dataset finder brings you back here.

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