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Loss of Alg13 Impairs Postnatal GABAergic Cortical Interneuron Development

GSE302303 Mus musculus (mouse) RNA-seq (gene expression) 18 samples Submitted 2026/01/05 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 18 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
18 samples: 6 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
Abnormal development of cortical neurons may be closely associated with the presence of neurological disorders, and deletion of the Alg13 gene was strongly associated with epilepsy susceptibility and seizure severity in mice. And the deletion of Alg13 gene is also found in some epileptic patients, which may prove that Alg13 gene plays a role in the development of cortical interneurons.
Read the full summary ↓
Methods We used immunofluorescence to observe the effects of Alg13 deletion on the distribution and migration of interneurons in the cerebral cortex of postnatal mice, and applied transcriptome sequencing to identify neuronal developmental genes and verified the results using RT-qPCR. Results Alg13 deletion plays a key role in the spatiotemporal distribution of cortical interneurons in the mouse cerebral cortex by establishing an Alg13-deficient mouse model by immunofluorescence, and that the migratory ability of interneuron subtypes in mice was also significantly inhibited by Alg13 deletion, which may increase the susceptibility to epilepsy and the severity of seizures. By transcriptome sequencing as well as RT-qPCR we verified three genes associated with inhibitory interneuron development, Ndn, Dynlt1b, and C3. Conclusions Abnormal development of interneurons may be regulated by Alg13, thus causing epilepsy-related disorders, which will help to further understand the pathogenesis and mechanisms of epilepsy, with a view to providing experimental basis and new therapeutic targets for the clinical application of controlling Alg13-associated refractory seizures, and enriching the understanding of the regulatory mechanisms of interneuron development.
This dataset
Open GSE302303 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE302303_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1289120. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗
Study design
6 conditions, mostly in triplicate
Alg13 knockout mice 1 day after born ×3 Alg13 knockout mice 30 day after born ×3 Alg13 knockout mice 7 day after born ×3 wild type mice 1 day after born ×3 wild type mice 30 day after born ×3 wild type mice 7 day after born ×3

Supports a case/control comparison: 9 samples read as cases, 9 as controls.

How we read this: 6 replicated groups read from 18 sample titles; they account for 18 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.