GEO series
Transcriptomic Profiling of CD66b+ Neutrophils from Healthy Controls and Cardiac Surgery Patients with Vasoplegic and Septic Shock Syndromes Across Multiple Timepoints
GSE302306
Homo sapiens (human)
RNA-seq (gene expression)
68 samples
Submitted 2026/05/14
Platform GPL21290
At a glance
RNA-seq (gene expression) · human · 68 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Samples
- 68 samples: 68 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Neutrophil Transcriptomic Changes in Severe Sterile Vasoplegic Syndrome Resemble a Distinct Molecular Subtype of Septic ShockShock (Augusta, Ga.) · 2026
Summary (written by the submitters)
Background: The systemic response to inflammation involves massive reorganization of gene expression which contributes to protection from infection but can also precipitate septic shock and organ failure. Understanding the early molecular events of systemic inflammation in sepsis is complicated by variance in both host-pathogen interactions and responses over time.
Read the full summary ↓
Vasoplegic syndrome after cardiac surgery resembles septic shock but lacks pathogen-related variables and proceeds over a defined, reproducible time course. Analysis of a single leukocyte cell type further enhances signal-to-noise. We hypothesized that human neutrophil transcriptomic changes in sterile vasoplegic syndrome would resemble neutrophil transcriptomic changes in one or more molecular subtypes of septic shock. Results: We enrolled seven patients with vasoplegic syndrome after cardiac surgery, eight cardiac surgical controls, and 12 patients with septic shock. In principal component analysis, isolated neutrophil transcriptomes from surgical patients clustered together by timepoint and demonstrated core dynamic gene expression changes in response to cardiac surgery that were independent of outcome. Additionally, among vasoplegic syndrome patients, two marked and consistent neutrophil transcriptomic phenotypes were observed and associated with distinct clinical phenotypes. A subset of dynamic expression changes in severe vasoplegic syndrome which were not seen in surgical controls instead resembled expression in patients with septic shock characterized by increased MMP8 mRNA. Similar results were obtained for plasma cytokine levels. Conclusions: Dynamic gene expression in neutrophils from patients with severe vasoplegic syndrome includes both expression changes in response to surgery, and development of a phenotype resembling septic shock with elevated MMP8 expression. Molecular events in severe vasoplegic syndrome may provide insights into the early mechanisms driving both septic and sterile inflammatory shock. Keywords: Sepsis, Surgical Trauma, Traumatic Injury
Published in
Siletz AE, Kelly-Scumpia KM, Scumpia PO et al.
· Shock (Augusta, Ga.) 2026
· PMID 41879344
· doi:10.1097/SHK.0000000000002827
This dataset
Download · which file do I need?
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
1 · Just have a look
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
2 · Re-analyse it
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE302306_RAW.tar (it can run to tens of gigabytes).
3 · Start from the raw reads
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1289838. Searching any of these in the dataset finder brings you back here.
Study design
68 samples, each with a distinct title — no replicated groups
Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.
How we read this: Read from 68 sample titles: 68 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
Samples in this study
- GSM9101509 CD66b+ neutrophils from healthy control patient 1 (32y female)
- GSM9101510 CD66b+ neutrophils from healthy control patient 10 (52y male)
- GSM9101511 CD66b+ neutrophils from healthy control patient 11 (66y female)
- GSM9101512 CD66b+ neutrophils from healthy control patient 12 (36y male)
- GSM9101513 CD66b+ neutrophils from healthy control patient 13 (55y male)
- GSM9101514 CD66b+ neutrophils from surgical control patient 16A at preoperative (66y female)
- GSM9101515 CD66b+ neutrophils from surgical control patient 16B at 0hr post-cpb (66y female)
- GSM9101516 CD66b+ neutrophils from surgical control patient 16C at 24hr post-cpb (66y female)
- GSM9101517 CD66b+ neutrophils from severe vasoplegic syndrome patient 17A at preoperative (28y male)
- GSM9101518 CD66b+ neutrophils from severe vasoplegic syndrome patient 17B at 0hr post-cpb (28y male)
- GSM9101519 CD66b+ neutrophils from severe vasoplegic syndrome patient 17C at 24hr post-cpb (28y male)
- GSM9101520 CD66b+ neutrophils from surgical control patient 20A.1 at preoperative (59y female)
- GSM9101521 CD66b+ neutrophils from surgical control patient 20B at 0hr post-cpb (59y female)
- GSM9101522 CD66b+ neutrophils from surgical control patient 20C at 24hr post-cpb (59y female)
- GSM9101523 CD66b+ neutrophils from mild vasoplegic syndrome patient 21A at preoperative (55y male)
- GSM9101524 CD66b+ neutrophils from mild vasoplegic syndrome patient 21B at 0hr post-cpb (55y male)
- GSM9101525 CD66b+ neutrophils from mild vasoplegic syndrome patient 21C at 24hr post-cpb (55y male)
- GSM9101526 CD66b+ neutrophils from severe vasoplegic syndrome patient 22A at preoperative (48y female)
- GSM9101527 CD66b+ neutrophils from severe vasoplegic syndrome patient 22B at 0hr post-cpb (48y female)
- GSM9101528 CD66b+ neutrophils from severe vasoplegic syndrome patient 22C at 24hr post-cpb (48y female)
- GSM9101529 CD66b+ neutrophils from severe vasoplegic syndrome patient 23A at preoperative (66y male)
- GSM9101530 CD66b+ neutrophils from severe vasoplegic syndrome patient 23B at 0hr post-cpb (66y male)
- GSM9101531 CD66b+ neutrophils from severe vasoplegic syndrome patient 23C at 24hr post-cpb (66y male)
- GSM9101532 CD66b+ neutrophils from surgical control patient 26A at preoperative (67y male)
- GSM9101533 CD66b+ neutrophils from surgical control patient 26B at 0hr post-cpb (67y male)
- GSM9101534 CD66b+ neutrophils from surgical control patient 26C at 24hr post-cpb (67y male)
- GSM9101535 CD66b+ neutrophils from surgical control patient 27A at preoperative (83y male)
- GSM9101536 CD66b+ neutrophils from surgical control patient 27B at 0hr post-cpb (83y male)
- GSM9101537 CD66b+ neutrophils from surgical control patient 27C at 24hr post-cpb (83y male)
- GSM9101538 CD66b+ neutrophils from mmp8+ septic shock patient 29 (88y male)
- GSM9101539 CD66b+ neutrophils from surgical control patient 30A at preoperative (77y female)
- GSM9101540 CD66b+ neutrophils from surgical control patient 30B at 0hr post-cpb (77y female)
- GSM9101541 CD66b+ neutrophils from surgical control patient 30C at 24hr post-cpb (77y female)
- GSM9101542 CD66b+ neutrophils from severe vasoplegic syndrome patient 31A at preoperative (46y male)
- GSM9101543 CD66b+ neutrophils from severe vasoplegic syndrome patient 31B at 0hr post-cpb (46y male)
- GSM9101544 CD66b+ neutrophils from severe vasoplegic syndrome patient 31C at 24hr post-cpb (46y male)
- GSM9101545 CD66b+ neutrophils from mixed cardiogenic and vasoplegic shock patient 32A at preoperative (57y female)
- GSM9101546 CD66b+ neutrophils from mixed cardiogenic and vasoplegic shock patient 32B at 0hr post-cpb (57y female)
- GSM9101547 CD66b+ neutrophils from mixed cardiogenic and vasoplegic shock patient 32C at 24hr post-cpb (57y female)
- GSM9101548 CD66b+ neutrophils from mmp8+ septic shock patient 35 (76y female)
+ 28 more — browse all 68 samples with per-sample file links →
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Share this dataset
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.