GEO series

Loss of Hepatocyte PI3Ka Protects Mice from Hepatocellular Carcinoma in association with altered lipid metabolism gene expression

GSE302836 Mus musculus (mouse) RNA-seq (gene expression) 42 samples Submitted 2026/04/30 Platform GPL24247

At a glance

RNA-seq (gene expression) · mouse · 42 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 42 samples: 42 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Loss of hepatocyte PI3Kα reduces hepatocellular carcinoma and hepatocyte proliferation in association with altered lipid metabolismJHEP reports : innovation in hepatology · 2026

Summary (written by the submitters)

Hepatocellular Carcinoma (HCC) is a disease with an increasing incidence and a high mortality rate; therefore, targeted therapies for HCC are urgently needed. PI3K-AKT-mTORC1 signaling is frequently induced in solid tumors and is associated with tumor progression and with the most aggressive type of HCC. However, complete inhibition of all PI3K isoforms is unlikely to achieve a favorable therapeutic index for HCC treatment due to on-target side effects.

Read the full summary ↓

Here, we investigate the role of hepatocyte PI3Ka in HCC using conditional knockout mice. We found that PI3Ka in the hepatocyte is dispensable for AKT phosphorylation in HCC and in normal liver, and hepatocytes during compensatory proliferation following administration of the hepatocarcinogen. We have also found that AKT phosphorylation induced by hepatocyte growth factor (HGF) and epidermal growth factor (EGF) is mediated by redundant PI3Ka and PI3Kb activities. Nonetheless, mice lacking hepatocyte PI3Ka showed reduced HCC proliferation and reduced hepatocyte proliferation induced by DEN. This phenotype was associated with a gene expression signature indicating altered lipid metabolism and reduced formation of lipid droplets. Together, these results indicate that PI3Ka is a promising target for the treatment of HCC.

Published in

Loss of hepatocyte PI3Kα reduces hepatocellular carcinoma and hepatocyte proliferation in association with altered lipid metabolism

Becattini B, Sardi C, Edelbroek B et al. · JHEP reports : innovation in hepatology 2026 · PMID 41997233 · doi:10.1016/j.jhepr.2026.101847

This dataset

Open GSE302836 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE302836_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1292245 and SRA study SRP601323. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41997233) ↗

Study design

42 samples, each with a distinct title — no replicated groups

Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Read from 42 sample titles: 42 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM9111932 PI3Ka F/F mouse 1, fed HFD, non-tumor liver tissue
GSM9111933 PI3Ka F/F mouse 2, fed HFD, non-tumor liver tissue
GSM9111934 PI3Ka F/F mouse 3, fed HFD, non-tumor liver tissue
GSM9111935 PI3Ka F/F mouse 4, fed HFD, non-tumor liver tissue
GSM9111936 PI3Ka F/F mouse 5, fed HFD, non-tumor liver tissue
GSM9111937 PI3Ka F/F mouse 6, fed HFD, non-tumor liver tissue
GSM9111938 PI3Ka F/F mouse 7, fed HFD, non-tumor liver tissue
GSM9111939 PI3Ka F/F mouse 8, fed HFD, HCC
GSM9111940 PI3Ka F/F mouse 9, fed HFD, HCC
GSM9111941 PI3Ka F/F mouse 10, fed HFD, HCC
GSM9111942 PI3Ka F/F mouse 11, fed HFD, HCC
GSM9111943 PI3Ka F/F mouse 12, fed HFD, HCC
GSM9111944 PI3Ka F/F mouse 13, fed HFD, HCC
GSM9111945 PI3Ka F/F mouse 14, fed HFD, HCC
GSM9111946 PI3Ka HEP mouse 15, fed HFD, non-tumor liver tissue
GSM9111947 PI3Ka HEP mouse 16, fed HFD, non-tumor liver tissue
GSM9111948 PI3Ka HEP mouse 17, fed HFD, non-tumor liver tissue
GSM9111949 PI3Ka HEP mouse 18, fed HFD, non-tumor liver tissue
GSM9111950 PI3Ka HEP mouse 19, fed HFD, non-tumor liver tissue
GSM9111951 PI3Ka HEP mouse 20, fed HFD, non-tumor liver tissue
GSM9111952 PI3Ka HEP mouse 21, fed HFD, non-tumor liver tissue
GSM9111953 PI3Ka HEP mouse 22, fed HFD, HCC
GSM9111954 PI3Ka HEP mouse 23, fed HFD, HCC
GSM9111955 PI3Ka HEP mouse 24, fed HFD, HCC
GSM9111956 PI3Ka HEP mouse 25, fed HFD, HCC
GSM9111957 PI3Ka HEP mouse 26, fed HFD, HCC
GSM9111958 PI3Ka HEP mouse 27, fed HFD, HCC
GSM9111959 PI3Ka HEP mouse 28, fed HFD, HCC
GSM9111960 PI3Ka F/F mouse 29, fed Chow, non-tumor liver tissue
GSM9111961 PI3Ka F/F mouse 30, fed Chow, non-tumor liver tissue
GSM9111962 PI3Ka F/F mouse 31, fed Chow, non-tumor liver tissue
GSM9111963 PI3Ka F/F mouse 32, fed Chow, non-tumor liver tissue
GSM9111964 PI3Ka F/F mouse 33, fed Chow, non-tumor liver tissue
GSM9111965 PI3Ka F/F mouse 34, fed Chow, non-tumor liver tissue
GSM9111966 PI3Ka F/F mouse 35, fed Chow, non-tumor liver tissue
GSM9111967 PI3Ka HEP mouse 36, fed Chow, non-tumor liver tissue
GSM9111968 PI3Ka HEP mouse 37, fed Chow, non-tumor liver tissue
GSM9111969 PI3Ka HEP mouse 38, fed Chow, non-tumor liver tissue
GSM9111970 PI3Ka HEP mouse 39, fed Chow, non-tumor liver tissue
GSM9111971 PI3Ka HEP mouse 40, fed Chow, non-tumor liver tissue

+ 2 more — browse all 42 samples with per-sample file links →

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Search all mouse RNA-seq datasets in GEO →

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