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Synergistic Effects of Red Light and Direct Current Electric Fields on Neurite Growth in 3D Neural Cell Cultures

GSE303050 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2025/07/19 Platform GPL34284
Summary
Both red-light illumination and direct-current electric fields (dcEFs) have independently been shown to promote neurite outgrowth in two-dimensional (2D) neural cell cultures. However, their combined effects in a three-dimensional (3D) culture environment remain unexplored. In this study, we examined the synergistic effects of red light and dcEFs on neurite extension in human neuroblastoma (SH-SY5Y) and mouse neuroblastoma (N2a) cells embedded within a 3D collagen gel matrix. Our results demonstrated that optimized dcEF stimulation and red-light exposure significantly enhanced neurite elongation and directional alignment. RNA sequencing further identified Neuropeptide Y (NPY) and its receptors as key mediators of these effects. Moreover, electrophysiological assessments revealed that neurons subjected to the combined stimulation exhibited enhanced functional maturation. These findings provide compelling evidence supporting the potential applications of red light and dcEFs in neural regeneration and repair.
Published in
Combined effects of red light and direct-current electric fields on neurite growth in 3D neural cell cultures
Kao YC, Huang HB, Lee CH · Scientific reports 2025 · PMID 41318831 · doi:10.1038/s41598-025-29835-0
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Also filed as BioProject PRJNA1293410 and SRA study SRP601836. Searching any of these in the dataset finder brings you back here.

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