A critical role of necroptosis in the oil cyst formation associated with autologous fat transplantation

Autologous fat grafting is limited by unpredictable long-term outcomes, with oil cyst formation being a major complication whose molecular mechanisms remain elusive. We established a novel surgically-induced mouse model demonstrating that ischemia duration time as the primary driver of oil cyst pathogenesis. Through RNA-sequencing of viable grafts (n=5) versus oil cysts (n=5), we identified the activation of RIPK3-MLKL signaling axis, revealing necroptosis-related pathways as critical molecular divergences.