← BioTransfer GEO Dataset Finder
GEO series

JARID2 Inhibition Reprograms Human Hematopoietic Progenitor Cells To Enhance Bone Marrow Transplantation

GSE303350 Homo sapiens (human) ChIP-seq / ATAC-seq (genome binding and open chromatin) 88 samples Submitted 2025/09/03 Platform GPL24676Platform GPL34284
At a glance

ChIP-seq / ATAC-seq (genome binding and open chromatin) · human · 88 samples

What it is
ChIP-seq / ATAC-seq (genome binding and open chromatin)Where proteins bind the genome, or which regions are open and active.
Organism
Homo sapiens (human)
Samples
88 samples: 34 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
JARID2 Inhibition Reprograms Human Hematopoietic Progenitor Cells To Enhance Bone Marrow TransplantationbioRxiv : the preprint server for biology · 2025
Summary (written by the submitters)
Hematopoietic stem cell transplantation is a common treatment for many blood disorders. Umbilical cord blood (UCB) serves as a valuable source of hematopoietic stem and progenitor cells (HSPCs), particularly for patients lacking a matched donor. However, the limited number of repopulating cells in UCB units restricts its clinical utility.
Read the full summary ↓
Here, we demonstrate that JARID2 knockdown, distinct from EZH2 loss, increases both the number and functionality of human UCB-derived HSPCs in vitro and in vivo. After ex vivo culture, these functionally enhanced HSPCs were identified as CD34⁺CD90⁺EPCR⁺CD49f⁻CD71⁻. Mechanistically, JARID2 knockdown promotes a quiescent, long-term self-renewal gene expression program by upregulating STAT1 and MHC class II. Notably, it also confers HSC-like potential to multipotent progenitors (MPPs), enhancing their regenerative capacity. These findings support JARID2 inhibition as a safe and reversible strategy to expand functional HSPCs from UCB ex vivo, potentially increasing access to this life-saving therapy for a broader patient population.
Published in
JARID2 Inhibition Reprograms Human Hematopoietic Progenitor Cells To Enhance Bone Marrow Transplantation
Han W, Bjeije H, Celik H et al. · bioRxiv : the preprint server for biology 2025 · PMID 40950155 · doi:10.1101/2025.09.03.672211
This dataset
Open GSE303350 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE303350_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Look for peak files (.bed, .narrowPeak) and signal tracks (.bigWig) among the supplementary files; the raw reads are on ENA.

Also filed as BioProject PRJNA1294881 and SRA study SRP602768. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40950155) ↗
Study design
34 conditions, mostly in duplicate
GFP+CD34+CD90+EPCR+CD49f-CD71-, IgG, C&T, ×4 Sorted GFP+CD34+CD90+EPCR+CD49f-CD71-, shEZH2… ×3 Sorted GFP+CD34+CD90+EPCR+CD49f-CD71-, shJARI… ×3 Sorted GFP+CD34+CD90+EPCR+CD49f-CD71-, shREN,… ×3 Umbilical cord blood CD34+, IgG, WT, C&R, CB ×3 GFP+CD34+CD90+EPCR+CD49f-CD71-, H2AK119ub1, s… ×3 GFP+CD34+CD90+EPCR+CD49f-CD71-, H2AK119ub1, s… ×3 GFP+CD34+CD90+EPCR+CD49f-CD71-, H2AK119ub1, s… ×3 +34 more

Supports a between-group comparison across 80 samples.

How we read this: 34 replicated groups read from 88 sample titles; they account for 80 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 48 more — browse all 88 samples with per-sample file links →

Datasets on a similar topic

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.