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Matrix stiffness regulates telocyte differentiation into fibroblasts in vitro

GSE303624 Mus musculus Expression profiling by high throughput sequencing 3 samples Submitted 2026/02/13 Platform GPL34290
Summary
Primary telocytes from ankle joints of Efhd1CreER x Ai9 mice were purified by FACS based on tdTomato (tdT) expression. These tdT⁺ telocytes were cultured on collagen-coated CytoSoft® plates of varying stiffness (0.2, 8, and 64 kPa). After 24 hours, cells were harvested for bulk RNA sequencing using Illumina NovaSeq X Plus. The expression of telocyte markers and fibroblast/myofibroblast markers showed stiffness-dependent changes. Notably, stiff ECM induced a telocyte-to-myofibroblast transition, evidenced by downregulation of Efhd1 and Myoc and upregulation of myofibroblast markers(eg. Acta2 and Tagln). Additional analysis revealed significant changes in expression of Timp, Mmp, and Collagen genes. These findings suggest that ECM stiffness is a key regulator of telocyte fate and function at the PLV surface.
Published in
The Function of Efhd1 (+) Telocytes in the Synovial Lymphatic System and Inflammatory-Erosive Arthritis
Peng Y, Mark Kenney H, Kobryn A et al. · bioRxiv : the preprint server for biology 2026 · PMID 41279427 · doi:10.1101/2025.11.03.685859
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Also filed as BioProject PRJNA1295943 and SRA study SRP603458. Searching any of these in the dataset finder brings you back here.

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