← BioTransfer GEO Dataset Finder
GEO series

RNA-seq profiling of SEM B-ALL cells silenced for KMT2A::AFF1 fusion circular RNA.

GSE304109 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2026/07/30 Platform GPL30173
Summary
The t(4;11) translocation, resulting in the KMT2A::AFF1 fusion gene, is associated with a poor prognosis in pediatric and adult B-cell Acute Lymphoblastic Leukemia (B-ALL). Recently, KMT2A::AFF1 fusion circular RNAs (f-circRNAs) were identified by bioinformatics in B-ALL; however, their contribution to leukemia has not been explored. In this context, we identified a novel recurrent back-splicing junction joining the AFF1 exon 8 to KMT2A exon 2 (AK_8_2), which was recurrent in t(4;11)-positive B-ALL cell lines as well as in pediatric and adult B-ALL patients. To investigate its functional role, we performed the total RNA-seq profiling of SEM B-ALL cells harboring the t(4;11) translocation and knocked-down for AK_8_2 f-circRNA, thus identifying a set of up-regulated genes potentially relevant for cell metabolism and leukemogenesis.
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE304109_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1298929 and SRA study SRP604670. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 6 more — browse all 6 samples with per-sample file links →

Similar datasets

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.