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Effects of stable SpCas9 expression in affecting gene expression in DU145 cells

GSE304365 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2025/11/14 Platform GPL34284
Summary
SpCas9 has been broadly used in gene editing, while as a bacterial protein, its effects on mammalian cell behavior remains unclear. We established stable SpCas9 expression in a panel of mammalian cell lines and found stable SpCas9 expression could affect cell growth in vitro. For example, we observed that SpCas9 expression promoted DU145 cell proliferation. To understand underlying mechansims, we performed this RNA-Seq analysis in DU145-NC (negative control) and SpCas9 expressing cells, aiming to find how SpCas9 modulates gene expression that may contribute to its cell proliferation control.
Published in
Stable Cas9 expression regulates cell growth by facilitating mTORC2 activation
Yu L, Jin Y, Chen J et al. · Nucleic acids research 2025 · PMID 41020501 · doi:10.1093/nar/gkaf965
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Also filed as BioProject PRJNA1301122 and SRA study SRP606247. Searching any of these in the dataset finder brings you back here.

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