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Single-cell RNA-Seq profiling to characterize mammary tumor infiltrated immune cells with iron supplement during anti-PD-1 immunotherapy

GSE304458 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2026/02/10 Platform GPL34290
Summary
In previous study, we demonstrated that iron supplementation augmented the antitumor effect of PD-1 based immunotherapy in triple-negative breast cancer models. To gain a more profound understanding into whether and how iron plus PD-1 antibody combination influences tumor immune microenvironment to exert better therapeutic activity, in this study, we performed single cell RNA-sequencing with PIP-seq to profile and compare tumor infiltrated CD45+ cells derived from control, iron or PD-1 antibody alone, and combination treated E0771 tumors.
Published in
Iron diminishes immunosuppressive macrophages and enhances anti-PD-1 immunotherapy in breast cancer models
Wang Q, Adams EL, Poole RA et al. · Journal of experimental & clinical cancer research : CR 2026 · PMID 41673739 · doi:10.1186/s13046-026-03661-2
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Direct links to NCBI, no account and no request form: the whole study as GSE304458_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1301333 and SRA study SRP606362. Searching any of these in the dataset finder brings you back here.

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