← BioTransfer GEO Dataset Finder
GEO series

DNAJ-PKAc Induces Metabolic Rewiring and a Glutamine Dependency in Fibrolamellar HCC

GSE305096 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2026/05/15 Platform GPL24247
Summary
Background: Fibrolamellar carcinoma (FLC) is a pediatric and adolescent liver cancer that is characterized by a recurrent fusion of DNAJB1 and PRKACA, yielding a chimeric translated protein, DNAJ-PKAc. PRKACA encodes the catalytic subunit of protein kinase A (PKA), a regulator of cellular metabolism. Methods: We generated a syngeneic murine model of FLC, TIBx-DNAJ-PKAc. We utilized preclinical models of FLC and human specimens to characterize the metabolic and immune effects of DNAJ-PKAc. Results: DNAJ-PKAc induced a high glycolytic flux and glutamine dependence to support nucleotide metabolism and redox homeostasis. TIBx-DNAJ-PKAc tumors demonstrated reduced T cell infiltration with impaired T cell activation. Systemic administration of a glutamine antagonist reversed the immune-inactivated phenotype of TIBx-DNAJ-PKAc tumors and provided durable control in combination with immune checkpoint inhibitors (ICI). Conclusion: The presence of DNAJ-PKAc creates a vulnerability to the combination of glutamine antimetabolite and ICI therapy in FLC.
Published in
DNAJ-PKAc induces metabolic rewiring and enhanced glutamine flux in fibrolamellar HCC
Kamdar Z, Neumann E, Howe K et al. · Journal of hepatology 2026 · PMID 41205756 · doi:10.1016/j.jhep.2025.10.027
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE305096_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1304424 and SRA study SRP608238. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 6 more — browse all 6 samples with per-sample file links →

Similar datasets

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.