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Spatially Resolved Insights Into Fistulating Crohn’s Disease Pathogenesis - bulk RNA-Seq

GSE305631 Homo sapiens (human) RNA-seq (gene expression) 15 samples Submitted 2025/09/30 Platform GPL24676
At a glance

RNA-seq (gene expression) · human · 15 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
15 samples: 15 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Spatial fibroblast niches define Crohn's fistulaeNature · 2026
Summary (written by the submitters)
The aim of this study was to investigate how over expression of transcription factors alters the gene expression program of primary human colonic fibroblasts. Fibroblasts isolated from healthy donor biopsies were transduced with lentiviral vectors to over-express TWIST1 or OSR2, or with empty vector as a control, followed by bulk RNA sequencing. The goal was to identify transcriptional pathways and effector genes regulated by these factors, with a focus on extracellular matrix organization, morphogen signaling, and fibroblast functional states.
Published in
Spatial fibroblast niches define Crohn's fistulae
McGregor C, Qin X, Jagielowicz M et al. · Nature 2026 · PMID 41224999 · doi:10.1038/s41586-025-09744-y
This dataset
Open GSE305631 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE305631_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1307311 and SRA study SRP610689. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41224999) ↗
Study design
15 samples, each with a distinct title — no replicated groups

Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Read from 15 sample titles: 15 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

Search all human RNA-seq datasets in GEO →

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.