GEO series

Basal level of ATF4 promotes T cell readiness for activation-induced proliferation [CUT&Run]

GSE305890 Mus musculus (mouse) ChIP-seq / ATAC-seq (genome binding and open chromatin) 11 samples Submitted 2026/01/21 Platform GPL24247

At a glance

ChIP-seq / ATAC-seq (genome binding and open chromatin) · mouse · 11 samples

What it is: ChIP-seq / ATAC-seq (genome binding and open chromatin)Where proteins bind the genome, or which regions are open and active.
Organism: Mus musculus (mouse)
Samples: 11 samples
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Basal level of ATF4 promotes T cell readiness for activation-induced proliferationiScience · 2026

Summary (written by the submitters)

Stress responding elements such as eIF2α phosphorylation are either upregulated or required during late-phase T cell activation and differentiation. However, it is not well understood whether these elements are essential within the first 12-24 hours post-stimulation, a period when mitochondrial activation and metabolic reprogramming are critical.

Read the full summary ↓

Here, we demonstrate that T cell activation-induced mTOR and GCN2 phosphorylation leads to the upregulation of ATF4 protein as early as 12 hours after stimulation. This early induction of ATF4 has transcriptional activities that positively or negatively regulate stress response, signaling, and metabolism. Loss of Atf4 in T cells alters transcriptome dynamics, impairs amino acid transport and biosynthesis, and disrupts cellular adaptive responses to ER stress and oxidative stress. It also hinders cell growth, resulting in defective effector cell differentiation in vitro or in vivo in a chronic LCMV infection model. Our findings suggest that a basal level of ATF4 during the early phase of T cell activation enhances the preparedness of cells to cope with integrated nutritional, protein folding, and oxidative stresses during the activation course. This, in turn, protects cells from stress-related cell death or senescence.

Published in

Basal level of ATF4 promotes T cell readiness for activation-induced proliferation

Yi Y, Luo C, Wang J et al. · iScience 2026 · PMID 41550765 · doi:10.1016/j.isci.2025.114277

This dataset

Open GSE305890 in finder → Find more mouse ChIP / ATAC / CUT&Tag datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE305890_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Look for peak files (.bed, .narrowPeak) and signal tracks (.bigWig) among the supplementary files; the raw reads are on ENA.

Also filed as BioProject PRJNA1308124 and SRA study SRP610020. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41550765) ↗

Samples in this study

The sample list for this study is not cached yet. Press Load samples and it will be fetched from NCBI.

+ 11 more — browse all 11 samples with per-sample file links →

Datasets on a similar topic

GSE305975 Basal level of ATF4 promotes T cell readiness for activation-induced proliferation 29 samples shares atf4, activation-induced, proliferation, readiness, promotes, basal, level
GSE313359 Hippocampal estrogen levels, receptor types and epigenetics contribute to sex-specific memory-vulnerabilities to concurrent acute stresses [Cut & Run] 45 samples shares cut, level
GSE279042 Reprogramming of epidermal keratinocytes by PITX1 transforms the cutaneous cellular landscape and promotes wound healing [CUT&Tag] 30 samples shares cut, promotes
GSE300925 Lyn expression in macrophages promotes TLR activation and restricts proliferation in an isoform-independent manner 48 samples shares proliferation, promotes
GSE295816 Tissue factor pathway inhibitor promotes the migration, proliferation and colonization of MSCs by regulating CXCL12/CXCR4 signaling pathway 20 samples shares proliferation, promotes
GSE304211 H4K16ac contributes to chromatin compartment reorganization during mitotic and meiotic transitions [CUTnTAG] 128 samples shares cut
GSE297309 SKP1A-mediated degradation of PRC2 triggers activation of Polycomb-silenced gene [CUT&Tag] 108 samples shares cut
GSE289642 Noncanonical PRC2 chromatin association reveals a key role of EZHIP in intergenerational epigenetic memory transition [ChIP-Seq, Cut & Run] 107 samples shares cut

Search all mouse ChIP / ATAC / CUT&Tag datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.