GEO series

Astrocyte glucocorticoid receptor signaling restricts neuronal plasticity [RNA-seq]

GSE306262 Mus musculus (mouse) Single-cell RNA-seq 40 samples Submitted 2026/03/20 Platform GPL19057

At a glance

Single-cell RNA-seq · mouse · 40 samples

What it is: Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart. Read from the summary: GEO's own type field only says 'expression profiling by sequencing', so confirm it in the paper.
Organism: Mus musculus (mouse)
Samples: 40 samples: 12 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.

Summary (written by the submitters)

Sensory experience refines neural circuits during critical periods of postnatal development. Although neuronal activity is known to orchestrate the circuit wiring that underlies this process, the environmental cues that restrain developmental plasticity as animals mature are less clear. Here, we examine the experience-dependent maturation of the mouse primary visual cortex (V1) across postnatal development using paired single-cell transcriptomic and chromatin accessibility sequencing.

Read the full summary ↓

In addition to identifying the activity-dependent gene programs that emerge within each cortical cell type, we find that light exposure drives astrocyte maturation through cell type-specific recruitment of the glucocorticoid receptor (Nr3c1/GR) to chromatin. Astrocyte GR signaling activates an extensive gene regulatory program that is partially conserved in human brain development and promotes maturation processes that may trigger critical period closure. Collectively, these findings reveal that astrocyte GR signaling restricts neuronal plasticity. Glucocorticoid regulation of astrocyte maturation may also contribute to the effects of early-life stress across the brain, and the disruption of this process may increase susceptibility to neuropsychiatric disease.

This dataset

Open GSE306262 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE306262_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.

Also filed as BioProject PRJNA1310321 and SRA study SRP612553. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Study design

12 conditions, mostly in triplicate

RNA-seq on P21 DR V1 astrocyte GR-Con nuclei, ×4 RNA-seq on P21 DR V1 astrocyte GR-KO nuclei, ×4 RNA-seq on P28 DR V1 astrocyte GR-Con nuclei, ×4 RNA-seq on P28 DR V1 astrocyte GR-KO nuclei, ×4 RNA-seq on P14 DR V1 astrocyte GR-Con nuclei, ×3 RNA-seq on P14 NR V1 astrocyte GR-Con nuclei, ×3 RNA-seq on P14 DR V1 astrocyte GR-KO nuclei, ×3 RNA-seq on P14 NR V1 astrocyte GR-KO nuclei, ×3 +4 more

Supports a between-group comparison across 40 samples.

How we read this: 12 replicated groups read from 40 sample titles; they account for 40 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM9195953 RNA-seq on P14 DR V1 astrocyte GR-Con nuclei, replicate 1 RNA-seq on P14 DR V1 as…
GSM9195955 RNA-seq on P14 DR V1 astrocyte GR-Con nuclei, replicate 2 RNA-seq on P14 DR V1 as…
GSM9195956 RNA-seq on P14 DR V1 astrocyte GR-Con nuclei, replicate 3 RNA-seq on P14 DR V1 as…
GSM9195958 RNA-seq on P14 NR V1 astrocyte GR-Con nuclei, replicate 1 RNA-seq on P14 NR V1 as…
GSM9195959 RNA-seq on P14 NR V1 astrocyte GR-Con nuclei, replicate 2 RNA-seq on P14 NR V1 as…
GSM9195960 RNA-seq on P14 NR V1 astrocyte GR-Con nuclei, replicate 3 RNA-seq on P14 NR V1 as…
GSM9195962 RNA-seq on P14 DR V1 astrocyte GR-KO nuclei, replicate 1 RNA-seq on P14 DR V1 as…
GSM9195963 RNA-seq on P14 DR V1 astrocyte GR-KO nuclei, replicate 2 RNA-seq on P14 DR V1 as…
GSM9195965 RNA-seq on P14 DR V1 astrocyte GR-KO nuclei, replicate 3 RNA-seq on P14 DR V1 as…
GSM9195966 RNA-seq on P14 NR V1 astrocyte GR-KO nuclei, replicate 1 RNA-seq on P14 NR V1 as…
GSM9195968 RNA-seq on P14 NR V1 astrocyte GR-KO nuclei, replicate 2 RNA-seq on P14 NR V1 as…
GSM9195970 RNA-seq on P14 NR V1 astrocyte GR-KO nuclei, replicate 3 RNA-seq on P14 NR V1 as…
GSM9195971 RNA-seq on P21 DR V1 astrocyte GR-Con nuclei, replicate 1 RNA-seq on P21 DR V1 as…
GSM9195973 RNA-seq on P21 DR V1 astrocyte GR-Con nuclei, replicate 2 RNA-seq on P21 DR V1 as…
GSM9195974 RNA-seq on P21 DR V1 astrocyte GR-Con nuclei, replicate 3 RNA-seq on P21 DR V1 as…
GSM9195975 RNA-seq on P21 DR V1 astrocyte GR-Con nuclei, replicate 4 RNA-seq on P21 DR V1 as…
GSM9195977 RNA-seq on P21 NR V1 astrocyte GR-Con nuclei, replicate 1 RNA-seq on P21 NR V1 as…
GSM9195978 RNA-seq on P21 NR V1 astrocyte GR-Con nuclei, replicate 2 RNA-seq on P21 NR V1 as…
GSM9195980 RNA-seq on P21 NR V1 astrocyte GR-Con nuclei, replicate 3 RNA-seq on P21 NR V1 as…
GSM9195981 RNA-seq on P21 DR V1 astrocyte GR-KO nuclei, replicate 1 RNA-seq on P21 DR V1 as…
GSM9195983 RNA-seq on P21 DR V1 astrocyte GR-KO nuclei, replicate 2 RNA-seq on P21 DR V1 as…
GSM9195984 RNA-seq on P21 DR V1 astrocyte GR-KO nuclei, replicate 3 RNA-seq on P21 DR V1 as…
GSM9195986 RNA-seq on P21 DR V1 astrocyte GR-KO nuclei, replicate 4 RNA-seq on P21 DR V1 as…
GSM9195987 RNA-seq on P21 NR V1 astrocyte GR-KO nuclei, replicate 1 RNA-seq on P21 NR V1 as…
GSM9195989 RNA-seq on P21 NR V1 astrocyte GR-KO nuclei, replicate 2 RNA-seq on P21 NR V1 as…
GSM9195990 RNA-seq on P21 NR V1 astrocyte GR-KO nuclei, replicate 3 RNA-seq on P21 NR V1 as…
GSM9195991 RNA-seq on P28 DR V1 astrocyte GR-Con nuclei, replicate 1 RNA-seq on P28 DR V1 as…
GSM9195993 RNA-seq on P28 DR V1 astrocyte GR-Con nuclei, replicate 2 RNA-seq on P28 DR V1 as…
GSM9195994 RNA-seq on P28 DR V1 astrocyte GR-Con nuclei, replicate 3 RNA-seq on P28 DR V1 as…
GSM9195996 RNA-seq on P28 DR V1 astrocyte GR-Con nuclei, replicate 4 RNA-seq on P28 DR V1 as…
GSM9195997 RNA-seq on P28 NR V1 astrocyte GR-Con nuclei, replicate 1 RNA-seq on P28 NR V1 as…
GSM9195999 RNA-seq on P28 NR V1 astrocyte GR-Con nuclei, replicate 2 RNA-seq on P28 NR V1 as…
GSM9196000 RNA-seq on P28 NR V1 astrocyte GR-Con nuclei, replicate 3 RNA-seq on P28 NR V1 as…
GSM9196001 RNA-seq on P28 DR V1 astrocyte GR-KO nuclei, replicate 1 RNA-seq on P28 DR V1 as…
GSM9196003 RNA-seq on P28 DR V1 astrocyte GR-KO nuclei, replicate 2 RNA-seq on P28 DR V1 as…
GSM9196004 RNA-seq on P28 DR V1 astrocyte GR-KO nuclei, replicate 3 RNA-seq on P28 DR V1 as…
GSM9196006 RNA-seq on P28 DR V1 astrocyte GR-KO nuclei, replicate 4 RNA-seq on P28 DR V1 as…
GSM9196007 RNA-seq on P28 NR V1 astrocyte GR-KO nuclei, replicate 1 RNA-seq on P28 NR V1 as…
GSM9196009 RNA-seq on P28 NR V1 astrocyte GR-KO nuclei, replicate 2 RNA-seq on P28 NR V1 as…
GSM9196010 RNA-seq on P28 NR V1 astrocyte GR-KO nuclei, replicate 3 RNA-seq on P28 NR V1 as…

Datasets on a similar topic

GSE306265 Astrocyte glucocorticoid receptor signaling restricts neuronal plasticity 106 samples shares glucocorticoid, plasticity, astrocyte, restricts, receptor, neuronal
GSE306261 Astrocyte glucocorticoid receptor signaling restricts neuronal plasticity [CUT&RUN] 50 samples shares glucocorticoid, plasticity, astrocyte, restricts, receptor, neuronal
GSE295530 MeCP2 stabilizes the glucocorticoid system in active chromatin regions [RNA-seq] 723 samples shares glucocorticoid, rna-seq
GSE248864 Hepatic Glucocorticoid Receptor (GR) drives the diurnal adaptation to Caloric Restriction 90 samples shares glucocorticoid, receptor
GSE270144 Disabling leading and lagging strand histone transmission results in parental histones loss and reduced cell plasticity and viability [RNA-Seq] 60 samples shares plasticity, rna-seq
GSE299346 Mecp2 stabilizes the glucocorticoid system in active chromatin regions [scRNA-Seq] 36 samples shares glucocorticoid, rna-seq
GSE263737 Bi-directional immunosuppressive interactions between astrocytes and glioblastoma cells identified by barcoded viral tracing [Mouse Bulk RNA-Seq Dataset of Monocytes, T Cells and Astrocytes 5OLJ] 33 samples shares astrocyte, rna-seq
GSE296482 A cell-intrinsic glucocorticoid biosynthesis and sensing circuit maintains homeostatic Th17 cell state [RNA-seq] 31 samples shares glucocorticoid, rna-seq

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