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Perturbational single-cell RNA sequencing of 104 compounds across six immortalized and four primary cell lines

GSE306429 Homo sapiens Expression profiling by high throughput sequencing; Other 348 samples 2025/10/23 GPL24676GPL21697
Summary
we introduce a new perturbational scRNA-seq dataset that includes six immortalized lines (A549, A375, H1AE, HEK293T, HEP2G, and PC3) and four primary lines: CD8+ T-cells (CD8+), CD34+ hematopoietic progenitors (CD34+), primary adipocytes (PAD), and bronchial epithelial cells (HBEC). 104 compounds, including 88 from CMap, were tested across the 10 cell types in duplicate with library-matched controls, resulting in 1,737 scRNA-seq samples comprising a total of 1.26 M cells.
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