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Fosl1 is a transcriptional effector of BRAFV600E-driven intestinal tumorigenesis

GSE306906 Mus musculus (mouse) RNA-seq (gene expression) 27 samples Submitted 2025/10/13 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 27 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
27 samples: 9 × control normal tissue vs 8 × adenoma from BrafV637E mutant mouse vs 7 × Fosl1-/- mutant mouse vs 3 × adenoma from FTg-ON transgene mutant mouseGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Fosl1 is a transcriptional effector of BRAF (V600E)-driven intestinal tumorigenesisiScience · 2025
Summary (written by the submitters)
The serrated neoplasia pathway in an alternate route to colorectal cancer (CRC) development where BRAFV600E is the most common initiating genetic alteration. BRAFV600E-driven tumorigenesisrequires gene expression changes mediated by activation of the ERK MAPK signalling pathway. However, the key effectors of this process are elusive. Here, we identify the ERK-regulated transcription factor Fosl1 one such effector.
Read the full summary ↓
We show that Fosl1 is dispensable for the initiation ofBRAFV600E-driven serrated neoplasia in mice but promotes progression of the disease by regulatingthe expression of genes involved in inflammation, immunity, cell cycle control, fetal-likeprogramming, and gastric metaplasia. Notably, transgenic Fosl1 expression alone was sufficient to induce tumours with a BRAFV600E-like serrated morphology and transcriptional profile. Thesefindings reveal a mechanism through which oncogenic BRAF-driven ERK signalling reprograms transcription to drive serrated neoplasia.
Published in
Fosl1 is a transcriptional effector of BRAF (V600E)-driven intestinal tumorigenesis
Alam Z, Nightingale R, Lesmana A et al. · iScience 2025 · PMID 41323278 · doi:10.1016/j.isci.2025.113875
This dataset
Open GSE306906 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE306906_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1313885 and SRA study SRP616825. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41323278) ↗
Study design
9 × control normal tissue vs 8 × adenoma from BrafV637E mutant mouse vs 7 × Fosl1-/- mutant mouse vs 3 × adenoma from FTg-ON transgene mutant mouse

Supports a case/control comparison: 18 samples read as cases, 9 as controls.

How we read this: 4 replicated groups read from 27 sample titles; they account for 27 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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