GEO series

Aberrant mRNA Splicing and Impaired Hippocampal Neurogenesis in Grin2b Mutant Mice [grin2b_snRNAseq]

GSE306925 Mus musculus (mouse) Single-nucleus RNA-seq 32 samples Submitted 2026/01/07 Platform GPL24247

At a glance

Single-nucleus RNA-seq · mouse · 32 samples

What it is: Single-nucleus RNA-seqGene activity measured in individual nuclei (used when whole cells are hard to isolate, for example from frozen tissue).
Organism: Mus musculus (mouse)
Samples: 32 samples: 8 conditions, mostly with about 4 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Aberrant mRNA splicing and impaired hippocampal neurogenesis in Grin2b mutant miceiScience · 2026

Summary (written by the submitters)

NMDA (N-methyl-d-aspartate) receptor dysfunction is widely implicated in pathophysiology of autism spectrum disorder (ASD), a neurodevelopmental condition with a strong genetic basis. Here we investigated heterozygous mouse mutants carrying the ASD-linked C456Y mutation of Grin2b, a high-confidence ASD risk gene encoding the GluN2B subunit of the NMDA receptors.

Read the full summary ↓

Comprehensive transcriptomic analyses across multiple brain regions and postnatal ages revealed large-scale gene expression changes, particularly in pathways related to oxidative phosphorylation and ribosome/translation, suggesting brain-wide alteration of energy metabolism and protein synthesis in Grin2b+/C456Y mice. Starting from transcriptomic evidence, we additionally discovered widespread splicing abnormalities, and impaired hippocampal neurogenesis in Grin2b mutants. Interestingly, the underlying genes and the spatial and temporal patterns of transcriptomic changes in Grin2b+/C456Y mice differed substantially from those observed in mutant mice lacking Grin2a, which encodes the GluN2A subunit of the NMDA receptor but is a schizophrenia risk gene. These findings underscore the distinct role of Grin2b in brain development and function and reveal potential mechanisms by which Grin2b loss of function may lead to neurodevelopmental disorders.

Published in

Aberrant mRNA splicing and impaired hippocampal neurogenesis in Grin2b mutant mice

Farsi Z, Nicolella A, Simmons SK et al. · iScience 2026 · PMID 41675057 · doi:10.1016/j.isci.2026.114700

This dataset

Open GSE306925 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE306925_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.

Also filed as BioProject PRJNA1313934 and SRA study SRP616435. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41675057) ↗

Study design

8 conditions, mostly with about 4 replicates each

4-week STR Grin2b +/- snRNA-seq Sample ×5 4-week STR WT snRNA-seq Sample ×5 4-week HP Grin2b +/- snRNA-seq Sample ×4 4-week HP WT snRNA-seq Sample ×4 4-week TH Grin2b +/- snRNA-seq Sample ×4 4-week TH WT snRNA-seq Sample ×4 4-week PFC Grin2b +/- snRNA-seq Sample ×3 4-week PFC WT snRNA-seq Sample ×3

Supports a between-group comparison across 32 samples.

How we read this: 8 replicated groups read from 32 sample titles; they account for 32 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM9212594 4-week HP Grin2b +/- snRNA-seq Sample 1 4-week HP Grin2b +/- sn…
GSM9212595 4-week HP Grin2b +/- snRNA-seq Sample 2 4-week HP Grin2b +/- sn…
GSM9212596 4-week HP Grin2b +/- snRNA-seq Sample 3 4-week HP Grin2b +/- sn…
GSM9212597 4-week HP Grin2b +/- snRNA-seq Sample 4 4-week HP Grin2b +/- sn…
GSM9212598 4-week HP WT snRNA-seq Sample 1 4-week HP WT snRNA-seq …
GSM9212599 4-week HP WT snRNA-seq Sample 2 4-week HP WT snRNA-seq …
GSM9212600 4-week HP WT snRNA-seq Sample 3 4-week HP WT snRNA-seq …
GSM9212601 4-week HP WT snRNA-seq Sample 4 4-week HP WT snRNA-seq …
GSM9212602 4-week STR Grin2b +/- snRNA-seq Sample 1 4-week STR Grin2b +/- s…
GSM9212603 4-week STR Grin2b +/- snRNA-seq Sample 2 4-week STR Grin2b +/- s…
GSM9212604 4-week STR Grin2b +/- snRNA-seq Sample 3 4-week STR Grin2b +/- s…
GSM9212605 4-week STR Grin2b +/- snRNA-seq Sample 4 4-week STR Grin2b +/- s…
GSM9212606 4-week STR Grin2b +/- snRNA-seq Sample 5 4-week STR Grin2b +/- s…
GSM9212607 4-week STR WT snRNA-seq Sample 1 4-week STR WT snRNA-seq…
GSM9212608 4-week STR WT snRNA-seq Sample 2 4-week STR WT snRNA-seq…
GSM9212609 4-week STR WT snRNA-seq Sample 3 4-week STR WT snRNA-seq…
GSM9212610 4-week STR WT snRNA-seq Sample 4 4-week STR WT snRNA-seq…
GSM9212611 4-week STR WT snRNA-seq Sample 5 4-week STR WT snRNA-seq…
GSM9212612 4-week TH Grin2b +/- snRNA-seq Sample 1 4-week TH Grin2b +/- sn…
GSM9212613 4-week TH Grin2b +/- snRNA-seq Sample 2 4-week TH Grin2b +/- sn…
GSM9212614 4-week TH Grin2b +/- snRNA-seq Sample 3 4-week TH Grin2b +/- sn…
GSM9212615 4-week TH Grin2b +/- snRNA-seq Sample 4 4-week TH Grin2b +/- sn…
GSM9212616 4-week TH WT snRNA-seq Sample 1 4-week TH WT snRNA-seq …
GSM9212617 4-week TH WT snRNA-seq Sample 2 4-week TH WT snRNA-seq …
GSM9212618 4-week TH WT snRNA-seq Sample 3 4-week TH WT snRNA-seq …
GSM9212619 4-week TH WT snRNA-seq Sample 4 4-week TH WT snRNA-seq …
GSM9212620 4-week PFC Grin2b +/- snRNA-seq Sample 1 4-week PFC Grin2b +/- s…
GSM9212621 4-week PFC Grin2b +/- snRNA-seq Sample 2 4-week PFC Grin2b +/- s…
GSM9212622 4-week PFC Grin2b +/- snRNA-seq Sample 3 4-week PFC Grin2b +/- s…
GSM9212623 4-week PFC WT snRNA-seq Sample 1 4-week PFC WT snRNA-seq…
GSM9212624 4-week PFC WT snRNA-seq Sample 2 4-week PFC WT snRNA-seq…
GSM9212625 4-week PFC WT snRNA-seq Sample 3 4-week PFC WT snRNA-seq…

Datasets on a similar topic

GSE312790 Aberrant mRNA Splicing and Impaired Hippocampal Neurogenesis in Grin2b Mutant Mice 133 samples shares grin2b, neurogenesis, hippocampal, aberrant, splicing, impaired, mutant
GSE312325 Aberrant mRNA Splicing and Impaired Hippocampal Neurogenesis in Grin2b Mutant Mice [RNAseq] 63 samples shares grin2b, neurogenesis, hippocampal, aberrant, splicing, impaired, mutant
GSE306924 Aberrant mRNA Splicing and Impaired Hippocampal Neurogenesis in Grin2b Mutant Mice [grin2b_bulkRNAseq] 38 samples shares grin2b, neurogenesis, hippocampal, aberrant, splicing, impaired, mutant
GSE237027 Transcriptomic changes in Grin2b-C456Y-mutant mice with NMDAR activation 23 samples shares grin2b, mutant
GSE237108 Transcriptome changes in Grin2b-C456Y-mutant mice with foot-shock stimulation 23 samples shares grin2b, mutant
GSE71485 Single-cell RNA-seq with Waterfall Reveals Molecular Cascades underlying Adult Hippocampal Neurogenesis 173 samples shares neurogenesis, hippocampal
GSE234317 The meninges host a unique compartment of regulatory T cells that bulwarks adult hippocampal neurogenesis 95 samples shares neurogenesis, hippocampal
GSE234316 The meninges host a unique compartment of regulatory T cells that bulwarks adult hippocampal neurogenesis [RNA-Seq] 87 samples shares neurogenesis, hippocampal

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.