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Genome-wide tongue epithelia transcriptomic profiles from K14-rtTA;TRE-FLBmi-1 + 4-nitroquinoline 1-oxide (4-NQO), 25 weeks (KrTB-N (25w)), KrTB+Doxycycline+4-NQO, 4 weeks (KrTB-DN (4w)), and KrTB+Doxycycline+4-NQO, 10 weeks (KrTB-DN (10w)).

GSE307391 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2025/12/09 Platform GPL24247
Summary
We developed a transgenic mouse model (truncated K14-rtTA; TRE/Bmi1, KrTB) containing a doxycycline- (dox) controlled, Tet-responsive element system to selectively overexpress BMI1 only in the basal epithelial stem cells (SCs) of the tongue. Here we used this transgenic mouse line to delineate BMI1 actions in early-stage oral squamous cell carcinoma (OSCC). We observed more oncogenic changes in mice with ectopic BMI1 expression after 4 weeks of 4-nitroquinoline 1-oxide (4-NQO) treatment. We detected increased proliferation and oxidative stress, as well as increased expression of multiple transcripts and proteins linked to human OSCCs, in murine tongue epithelia with high BMI1 expression during early carcinogen-induced tumorigenesis. Furthermore, increases in mRNAs encoding multiple metabolic targets, such as SLC16A3, PKM, and GPI1, were accelerated upon BMI1 overexpression in our 4-NQO model.
Published in
Key Early Changes in Oral Squamous Cell Carcinogenesis Are Accelerated by Ectopic BMI1 Expression
Baquero J, Tang XH, Galke D et al. · Cancer research communications 2026 · PMID 41385756 · doi:10.1158/2767-9764.CRC-25-0580
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Direct links to NCBI, no account and no request form: the whole study as GSE307391_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1320642 and SRA study SRP617768. Searching any of these in the dataset finder brings you back here.

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