GEO series

scRNA-seq of MHCII-KO and WT B cells during influenza infection

GSE307658 Mus musculus Other; Expression profiling by high throughput sequencing 4 samples Submitted 2026/02/21 Platform GPL24247

Summary

To evaluate the programming of B cells subsets that arise in the absence of CD4 T cell interactions we adoptively transferred wild-type (WT) and majorhistocompatibility class II (MHC-II) knockout B cells into WT hosts and infected the mice with influenza PR8. All activated responding transferred B cells were profiled at day 14, a time point when T cell independent memory responses arise, and scRNA-seq and BCR-seq performed to understand the transcriptional programming that is dependent on CD4 T cell interactions. We identified minimal differences between WT and KO B cells, with a diverse number of memory B cell clusters identified that were populated by cells fron both cohorts of mice. These data indicate that the memory B cells that form early, likely extrafollicularly, do not depend on CD4 T cells for their programming and diversity.

Published in

Transcriptional programming of early-forming memory B cells arises independently of cognate CD4+ T-cell interactions

Wiggins KJ, Williams ME, Hicks SL et al. · Journal of immunology (Baltimore, Md. : 1950) 2026 · PMID 42001515 · doi:10.1093/jimmun/vkag054

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Direct links to NCBI, no account and no request form: the whole study as GSE307658_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Whole study · RAW.tar ↓ Series matrix ↓ All supplementary files ↗ Raw reads on ENA ↗

Also filed as BioProject PRJNA1322470 and SRA study SRP618816. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 42001515) ↗

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