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ChIP-seq analysis of IRF1 binding in VSV-infected bone marrow-derived macrophages (BMDMs)

GSE308320 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 6 samples Submitted 2026/03/31 Platform GPL24247
Summary
This study examines the genome-wide binding profile of the transcription factor IRF1 in bone marrow-derived macrophages (BMDMs) infected with vesicular stomatitis virus (VSV) compared to uninfected controls. BMDMs were differentiated from mouse bone marrow cells via M-CSF stimulation and then infected with VSV (MOI = 1) for 4 hours or mock-treated. Chromatin immunoprecipitation (ChIP) was performed using an anti-IRF1 antibody, followed by next-generation sequencing (Illumina). Data analysis included alignment to the mm10 genome, peak calling, and coverage normalization to identify IRF1 binding sites under viral infection. These results provide insights into IRF1's regulatory role in antiviral immune responses.
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Direct links to NCBI, no account and no request form: the whole study as GSE308320_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1330187 and SRA study SRP621145. Searching any of these in the dataset finder brings you back here.

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