← BioTransfer GEO Dataset Finder
GEO series

Proteoglycofili are glycosaminoglycan-containing fibrillar components released by neutrophils to neutralize bacteria

GSE308441 Homo sapiens Expression profiling by high throughput sequencing 8 samples Submitted 2025/09/18 Platform GPL18573
Summary
Here we uncover that neutrophils release a previously unidentified fibrillar complex, named proteoglycofili (PGF) under hypoxic conditions or upon bacterial infection, using Shigella as a model. PGF are preferentially released by neutrophils over NETs upon bacterial infection. We demonstrated that PGF are released by living neutrophils, do not contain DNA and are mainly composed of granule proteins. We revealed that the fibrillar structure of PGF is sustained by two glycosaminoglycans (GAGs), hyaluronic acid and chondroitin sulfate, firstly identified here to be released by neutrophils. We demonstrated that PGF are potent antimicrobials, which degrade virulence factors of Shigella and block its growth, similarly with E. coli or Salmonella. We confirmed that GAGs were essential for PGF antimicrobial activity in vitro and in vivo. Beyond bacterial infections, our results strongly suggest that GAGs may be released by neutrophils in a broad range of inflammatory diseases to be further explored.
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE308441_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1330599 and SRA study SRP621785. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 8 more — browse all 8 samples with per-sample file links →

Similar datasets

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.