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Transcriptomic effects of miR-21-5p on preimplantation mouse blastocysts

GSE308541 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2026/06/15 Platform GPL34290
Summary
Preimplantation embryos cultured in vitro exhibit increased stress and apoptosis compared with in vivo development. miR-21-5p is a conserved, highly abundant microRNA in reproductive-tract extracellular vesicles and has been implicated in cell-survival pathways. To define its transcriptomic impact on early embryos, we performed RNA-seq on mouse blastocysts generated by IVF and cultured in KSOM with either miR-21-5p agomir (50 nM) or blank control. Each biological replicate consisted of five pooled blastocysts; n=4 replicates per group. Sequencing was carried out on an Illumina NovaSeq X Plus.
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Also filed as BioProject PRJNA1330846 and SRA study SRP622001. Searching any of these in the dataset finder brings you back here.

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