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BoNT/A Modulates Microglial Phagocytic Activity in the Hippocampus Revealed by Single-Cell RNA Sequencing

GSE308594 Mus musculus Expression profiling by high throughput sequencing 3 samples Submitted 2026/06/24 Platform GPL34328
Summary
To investigate the impact of Botulinum Neurotoxin A (BoNT/A) on hippocampal cellular landscapes, we performed single-cell RNA sequencing (scRNA-seq) on tissues from Saline-, MPTP-, and MPTP+BoNT/A-treated mice. We identified and annotated 10 major cell types through canonical marker gene expression. A focused sub-clustering analysis of microglia revealed 11 distinct subpopulations. Notably, KEGG pathway enrichment analysis identified three subclusters (1, 3, and 5) as phagocytosis-related microglia, enriched in pathways such as lysosome, phagosome, and FcγR-mediated phagocytosis. Our findings demonstrate that BoNT/A plays a definitive role in modulating microglial phagocytic activity in the hippocampus.
Published in
The complement C3-microglial axis in depression of Parkinson's disease: from mechanism to therapeutic intervention
Yin Q, Ding M, Tang Y et al. · EBioMedicine 2026 · PMID 42263400 · doi:10.1016/j.ebiom.2026.106325
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Also filed as BioProject PRJNA1331671 and SRA study SRP622312. Searching any of these in the dataset finder brings you back here.

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