GEO series
Temporal dynamics of collateral RNA cleavage by LbuCas13a in human cells
GSE308909
Homo sapiens
Expression profiling by high throughput sequencing
36 samples
2025/09/24
GPL34281
Summary
We have found that LbuCas13a exhibits collateral RNA cleavage activity in human cells after activation by the target RNA. Here we use total RNA sequencing to explore which RNAs are depleted by the collateral activity of LbuCas13a. ERCC spike-in RNAs were used for normalization between samples. This revealed near global depletion of mRNAs at 50 and 200 minutes after Cas13 protein and guide delivery. Mitochondrial and nuclear transcripts were not affected.
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Paper (PMID 41554880) ↗
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